IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Futterman, S.
Right arrow Articles by Kalina, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Futterman, S.
Right arrow Articles by Kalina, R. E.

Investigative Ophthalmology & Visual Science, Vol 14, 125-130, Copyright © 1975 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

A new, rapid fluorometric determination of retinol in serum

S Futterman, D Swanson and RE Kalina

A new procedure for the determination of retinol (vitamin A alcohol) in blood has been devised. It consists of direct measurement of the fluorescence of retinol (excitation 335 nm.; emission 458 nm.) in diluted serum, and is considerably more rapid than any previous method. Such measurements are feasible because the intensity of fluorescence of retinol bound to its transport protein (retinol-binding protein) is sufficiently high that other natural occurring fluorescent substances in blood do not interfere significantly. When the new method was compared to a conventional procedure employing extraction and chromatographic separation of retinol on columns of alumina, the correlation coefficient was 0.85.


This article has been cited by other articles:


Home page
 J. Nutr.Home page
N. E. Craft
Innovative Approaches to Vitamin A Assessment
J. Nutr., May 1, 2001; 131(5): 1626S - 1630.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the Association for Research in Vision and Ophthalmology