IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stein, P. C.
Right arrow Articles by Char, D. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stein, P. C.
Right arrow Articles by Char, D. H.

Investigative Ophthalmology & Visual Science, Vol 19, 302-305, Copyright © 1980 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Characterization of retinoblastoma immune complexes

PC Stein, M Christensen and DH Char

Immune complexes from retinoblastoma sera were characterized with molecular sieve chromatography, affinity chromatography, and polyacrylamide gel electrophoresis (PAGE). Retinoblastoma patients' sera had two well-defined peaks of immune complex activity after molecular sieve chromatography. These protein fractions had a molecular weight of approximately 1.6 x 10(5) and 2.0 x 10(6) daltons. Affinity chromatography with Sepharose 4B-protein A and analytical PAGE demonstrated that IgG was the predominant immunoglobulin in these immune compelxes. Immune complexes also had affinity for Sepharose- concanavalin A, indicating the glycoprotein nature of the antigen component.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the Association for Research in Vision and Ophthalmology