Investigative Ophthalmology & Visual Science, Vol 27, 1470-1477, Copyright © 1986 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Organization of collagen types I and V in the embryonic chicken cornea

DE Birk, JM Fitch and TF Linsenmayer

The distribution and organization of type I and type V collagens were studied in the embryonic chicken cornea using anti-collagen, type specific, monoclonal antibodies and immunoelectron microscopy. These studies were performed on lathyritic 17-day corneas treated at 4 degrees C or 37 degrees C. At the lower temperature, collagen fibril structure is disrupted; at the higher temperature, normal fibril structure is maintained. Corneas from non-lathyritic 17-day chick embryos, reacted at the two different temperatures, were studied for comparison. In Bowman's membrane, the thin (20 nm) fibrils were labelled by antibodies against both type I and type V collagen under all conditions studied. In the corneal stroma, the striated collagen fibrils (25 nm) were labelled with the antibodies against type I collagen in all cases, and by antibodies against type V collagen under conditions where fibril structure was disrupted. These results are consistent with the concept of heteropolymeric fibrils consisting of both type I and type V collagen molecules assembled such that the epitopes on the type V molecule are unavailable to antibody unless the fibrillar structure is disrupted. We suggest that the interaction of type V collagen with type I collagen may be responsible for the small diameter fibrils and the rigid control of fibril structure found in the cornea.

This article has been cited by other articles:

				K. Musselmann, B. Kane, B. Alexandrou, and J. R. Hassell Stimulation of Collagen Synthesis by Insulin and Proteoglycan Accumulation by Ascorbate in Bovine Keratocytes In Vitro Invest. Ophthalmol. Vis. Sci., December 1, 2006; 47(12): 5260 - 5266. [Abstract] [Full Text] [PDF]

				J. He and H. E. P. Bazan Synergistic Effect of Platelet-Activating Factor and Tumor Necrosis Factor-{alpha} on Corneal Myofibroblast Apoptosis. Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 883 - 891. [Abstract] [Full Text] [PDF]

				H. Chanut-Delalande, C. Bonod-Bidaud, S. Cogne, M. Malbouyres, F. Ramirez, A. Fichard, and F. Ruggiero Development of a Functional Skin Matrix Requires Deposition of Collagen V Heterotrimers Mol. Cell. Biol., July 1, 2004; 24(13): 6049 - 6057. [Abstract] [Full Text] [PDF]

				A. J. Quantock, C. Boote, V. Siegler, and K. M. Meek Collagen Organization in the Secondary Chick Cornea during Development Invest. Ophthalmol. Vis. Sci., January 1, 2003; 44(1): 130 - 136. [Abstract] [Full Text] [PDF]

				Y. Akimoto, N. Yamakawa, K. Furukawa, K. Kimata, H. Kawakami, and H. Hirano Changes in Distribution of the Long Form of Type XII Collagen during Chicken Corneal Development J. Histochem. Cytochem., June 1, 2002; 50(6): 851 - 862. [Abstract] [Full Text] [PDF]

				M. P. Beales, J. L. Funderburgh, J. V. Jester, and J. R. Hassell Proteoglycan Synthesis by Bovine Keratocytes and Corneal Fibroblasts: Maintenance of the Keratocyte Phenotype in Culture Invest. Ophthalmol. Vis. Sci., July 1, 1999; 40(8): 1658 - 1663. [Abstract] [Full Text] [PDF]

				H. Chanut-Delalande, A. Fichard, S. Bernocco, R. Garrone, D. J. S. Hulmes, and F. Ruggiero Control of Heterotypic Fibril Formation by Collagen V Is Determined by Chain Stoichiometry J. Biol. Chem., June 22, 2001; 276(26): 24352 - 24359. [Abstract] [Full Text] [PDF]