Investigative Ophthalmology & Visual Science, Vol 28, 1687-1692, Copyright © 1987 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Protein processing in lens intercellular junctions: cleavage of MP70 to MP38

J Kistler and S Bullivant
Department of Cell Biology, University of Auckland, New Zealand.

Membrane protein MP70 is a component specifically of lens fiber 16-17 nm junctions. SDS-PAGE analysis of membrane preparations made separately from the sheep lens equatorial region (outer cortex), inner cortex and central region (nucleus) showed abundant MP70 in the young fibers in the outer cortex and drastically reduced levels of MP70 in the older fibers deeper in the lens. MP70 was cleaved to MP38 and thereby lost the epitope to monoclonal anti MP70 (6-4-B2-C6, Kistler et al J Cell Biol 101:28, 1985). This cleavage is fiber age-related and is effected by a calcium-dependent, lens-endogenous protease.

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