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Investigative Ophthalmology & Visual Science, Vol 30, 2114-2117, Copyright © 1989 by Association for Research in Vision and Ophthalmology
ARTICLES AND REPORTS |
R Salceda
Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Mexico D.F.
Uptake of 45Ca was studied in isolated frog retinal pigment epithelial cells. 45Ca accumulation was found to be a saturable, temperature- dependent event. Kinetic analysis of this accumulation revealed two transport systems with apparent km of 2.0 and 0.3 mM. We found the presence of a Na-Ca exchanger mechanism that releases Ca2 under depolarized conditions. Light induced an increase of 45Ca uptake due to activation of the Na-K-ATPase and consequent decrease of extracellular potassium concentration.
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