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Investigative Ophthalmology & Visual Science, Vol 31, 2046-2050, Copyright © 1990 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Background-protein effects on fluorophotometric data

MF Barsotti, SP Bartels, RD Kamm and TF Freddo
Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge.

Fluorescent tracers are commonly used in fluorophotometric studies of ocular fluids and tissues that contain background protein. Background- protein concentrations were found to decrease or increase significantly the measure of fluorescence emitted from solutions containing sodium fluorescein, fluorescein-labeled dextran, or fluorescein-labeled horseradish peroxidase. The effect of background protein on fluorescence was expressed as a function of the specific fluorescent tracer, tracer concentration, and background-protein concentration; it can be corrected in the analysis of fluorophotometric data. Fluorophotometric studies--particularly those in which the background- protein level is expected to be abnormally high, such as postoperative and pathologic studies--may need to include either a data correction based on measured effects of background protein on tracer fluorescence or, in the case of clinical investigations, recognize at least the potential for a range of possible interpretations.




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Copyright © 1990 by the Association for Research in Vision and Ophthalmology