| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Investigative Ophthalmology & Visual Science, Vol 32, 1464-1470, Copyright © 1991 by Association for Research in Vision and Ophthalmology
ARTICLES AND REPORTS |
D Boyle, LF Tien, NG Cooper, V Shepherd and BJ McLaughlin
Department of Ophthalmology & Visual Sciences, University of Louisville School of Medicine, KY 40292.
A 175-kD mannose-specific receptor has been described in macrophages which appears to mediate pinocytosis and phagocytosis. A mannose- specific receptor has also been found on retinal pigment epithelial cells (RPE). Its role was examined in the phagocytosis of photoreceptor outer segments (ROS) by the RPE. All testing was done on cultured RPE cells challenged with fluorescein isothiocyanate (FITC)-labeled ROS for 4 hr at 37 degrees C. Total (internalized and bound) and phagocytized (internalized) ROS were quantified, and the effects of several experimental conditions on ROS phagocytosis were examined. Incubation of RPE cells in the presence of rabbit anti-serum (1:100) raised against the rat alveolar macrophage mannose receptor (anti-Mr) showed a 80% reduction in ROS phagocytosis compared with RPE in the presence of ROS alone. Anti-Mr preabsorbed with purified human mannose receptor protein (4 micrograms) showed no reduction in ROS phagocytosis. Incubation of RPE with preimmune serum showed no reduction in ROS phagocytosis. When FITC-labeled ROS (1.8 X 10(7)) were preincubated with purified mannose receptor, there was a 93% reduction in phagocytosis. Immunoblots of solubilized rat RPE microvillus membranes stained with the anti-Mr showed a single stained band at the 175-kD region, and immunolocalization studies showed specific labeling along RPE microvilli. These results suggest that a mannose-specific receptor is involved in retinal phagocytosis.
This article has been cited by other articles:
|
|
 |
|
  R. Li, A. Maminishkis, G. Zahn, D. Vossmeyer, and S. S. Miller Integrin {alpha}5{beta}1 Mediates Attachment, Migration, and Proliferation in Human Retinal Pigment Epithelium: Relevance for Proliferative Retinal Disease Invest. Ophthalmol. Vis. Sci., December 1, 2009; 50(12): 5988 - 5996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chen, E. Muckersie, M. Robertson, M. Fraczek, J. V. Forrester, and H. Xu Characterization of a Spontaneous Mouse Retinal Pigment Epithelial Cell Line B6-RPE07 Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3699 - 3706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sun, S. C. Finnemann, M. Febbraio, L. Shan, S. P. Annangudi, E. A. Podrez, G. Hoppe, R. Darrow, D. T. Organisciak, R. G. Salomon, et al. Light-induced Oxidation of Photoreceptor Outer Segment Phospholipids Generates Ligands for CD36-mediated Phagocytosis by Retinal Pigment Epithelium: A POTENTIAL MECHANISM FOR MODULATING OUTER SEGMENT PHAGOCYTOSIS UNDER OXIDANT STRESS CONDITIONS J. Biol. Chem., February 17, 2006; 281(7): 4222 - 4230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Chowers, Y. Kim, R. H. Farkas, T. L. Gunatilaka, A. S. Hackam, P. A. Campochiaro, S. C. Finnemann, and D. J. Zack Changes in Retinal Pigment Epithelial Gene Expression Induced by Rod Outer Segment Uptake Invest. Ophthalmol. Vis. Sci., July 1, 2004; 45(7): 2098 - 2106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. McHenry, Y. Liu, W. Feng, A. R. Nair, K. L. Feathers, X. Ding, A. Gal, D. Vollrath, P. A. Sieving, and D. A. Thompson MERTK Arginine-844-Cysteine in a Patient with Severe Rod-Cone Dystrophy: Loss of Mutant Protein Function in Transfected Cells Invest. Ophthalmol. Vis. Sci., May 1, 2004; 45(5): 1456 - 1463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Duncan, M. M. LaVail, D. Yasumura, M. T. Matthes, H. Yang, N. Trautmann, A. V. Chappelow, W. Feng, H. S. Earp, G. K. Matsushima, et al. An RCS-Like Retinal Dystrophy Phenotype in Mer Knockout Mice Invest. Ophthalmol. Vis. Sci., February 1, 2003; 44(2): 826 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Feng, D. Yasumura, M. T. Matthes, M. M. LaVail, and D. Vollrath Mertk Triggers Uptake of Photoreceptor Outer Segments during Phagocytosis by Cultured Retinal Pigment Epithelial Cells J. Biol. Chem., May 3, 2002; 277(19): 17016 - 17022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Finnemann and R. L. Silverstein Differential Roles of CD36 and {alpha}v{beta}5 Integrin in Photoreceptor Phagocytosis by the Retinal Pigment Epithelium J. Exp. Med., November 5, 2001; 194(9): 1289 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ikemoto, A. Fukuma, Y. Fujii, and H. Okuyama Lysosomal Enzyme Activities Are Decreased in the Retina and Their Circadian Rhythms Are Different from Those in the Pineal Gland of Rats Fed an {{alpha}}-Linolenic Acid-Restricted Diet J. Nutr., December 1, 2000; 130(12): 3059 - 3062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. D'Cruz, D. Yasumura, J. Weir, M. T. Matthes, H. Abderrahim, M. M. LaVail, and D. Vollrath Mutation of the receptor tyrosine kinase gene Mertk in the retinal dystrophic RCS rat Hum. Mol. Genet., March 1, 2000; 9(4): 645 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Anderson, G. S. Hageman, R. F. Mullins, M. Neitz, J. Neitz, S. Ozaki, K. T. Preissner, and L. V. Johnson Vitronectin Gene Expression in the Adult Human Retina Invest. Ophthalmol. Vis. Sci., December 1, 1999; 40(13): 3305 - 3315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. Zhao, M. L. Jin, S. He, C. Spee, S. J. Ryan, and D. R. Hinton A Distinct Integrin-Mediated Phagocytic Pathway for Extracellular Matrix Remodeling by RPE Cells Invest. Ophthalmol. Vis. Sci., October 1, 1999; 40(11): 2713 - 2723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Finnemann and E. Rodriguez-Boulan Macrophage and Retinal Pigment Epithelium Phagocytosis: Apoptotic Cells and Photoreceptors Compete for {alpha}v{beta}3 and {alpha}v{beta}5 Integrins, and Protein Kinase C Regulates {alpha}v{beta}5 Binding and Cytoskeletal Linkage J. Exp. Med., September 20, 1999; 190(6): 861 - 874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Finnemann, V. L. Bonilha, A. D. Marmorstein, and E. Rodriguez-Boulan Phagocytosis of rod outer segments by retinal pigment epithelial cells requires alpha vbeta 5 integrin for binding but not for internalization PNAS, November 25, 1997; 94(24): 12932 - 12937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Alliegro and L. A. Linz Novel Characteristics of a Myosin Isolated from Mammalian Retinal Pigment Epithelial and Endothelial Cells J. Biol. Chem., March 28, 1997; 272(13): 8759 - 8763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
AlexeyV. Ershov, WalterJ. Lukiw, and NicolasG. Bazan Selective Transcription Factor Induction in Retinal Pigment Epithelial Cells during Photoreceptor Phagocytosis J. Biol. Chem., November 8, 1996; 271(45): 28458 - 28462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ryeom, J. Sparrow, and R. Silverstein CD36 participates in the phagocytosis of rod outer segments by retinal pigment epithelium J. Cell Sci., January 2, 1996; 109(2): 387 - 395. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
