IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lavker, R. M.
Right arrow Articles by Sun, T. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lavker, R. M.
Right arrow Articles by Sun, T. T.

Investigative Ophthalmology & Visual Science, Vol 32, 1864-1875, Copyright © 1991 by Association for Research in Vision and Ophthalmology

ARTICLES AND REPORTS

Relative proliferative rates of limbal and corneal epithelia. Implications of corneal epithelial migration, circadian rhythm, and suprabasally located DNA-synthesizing keratinocytes

RM Lavker, G Dong, SZ Cheng, K Kudoh, G Cotsarelis and TT Sun
Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia.

An important element of the recently proposed limbal stem cell model is that corneal epithelial cells migrate centripetally. The driving force for this migration is unknown, although it has been suggested that limbal epithelium, proliferates at a higher rate than central corneal epithelium, thus creating a population pressure toward the central cornea. This hypothesis was tested by measuring the relative proliferative rates of limbal and central corneal epithelia using 3H- thymidine autoradiographic techniques. The results indicate that, in both the New Zealand white rabbit and SENCAR mouse, the labeling index (LI) of limbal epithelium is actually lower than that of central corneal epithelium. This difference in LI persists throughout the circadian rhythm cycle. These results suggest that population pressure per se cannot be responsible for the centripetal migration of corneal epithelium and raise the possibility that preferential desquamation of central corneal epithelium may "draw" peripheral cells toward the central cornea. In both epithelia, the LI peak precedes the mitotic index (MI) peak during circadian cycle by 4-6 hr. These data therefore are in close agreement with earlier results on several nonocular stratified epithelia but contradict an earlier suggestion that the LI and MI peaks of corneal epithelium coincide. Finally, although most of the 3H-thymidine incorporating cells in central cornea may appear to be suprabasally located, they are only partially displaced into the suprabasal compartment. In most cases, such cells are still connected with the basement membrane through a thin stalk of cytoplasm. Since corneal epithelium rests on an exceptionally flat and rigid substratum, an increase in cellular volume in DNA-synthesizing cells may not be tolerated well in an already crowded basal layer. This may explain why an unusually large proportion of DNA-synthesizing cells are expelled preferentially into either a "second tier basal layer" or into the suprabasal compartment.


This article has been cited by other articles:


Home page
 IOVSHome page
M. Krulova, K. Pokorna, A. Lencova, J. Fric, A. Zajicova, M. Filipec, J. V. Forrester, and V. Holan
A Rapid Separation of Two Distinct Populations of Mouse Corneal Epithelial Cells with Limbal Stem Cell Characteristics by Centrifugation on Percoll Gradient
Invest. Ophthalmol. Vis. Sci., September 1, 2008; 49(9): 3903 - 3908.
[Abstract] [Full Text] [PDF]

Home page
 Br J OphthalmolHome page
H Qi, D-Q Li, F Bian, E Y Chuang, D B Jones, and S C Pflugfelder
Expression of glial cell-derived neurotrophic factor and its receptor in the stem-cell-containing human limbal epithelium
Br J Ophthalmol, September 1, 2008; 92(9): 1269 - 1274.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. K. Swamynathan, J. Davis, and J. Piatigorsky
Identification of Candidate Klf4 Target Genes Reveals the Molecular Basis of the Diverse Regulatory Roles of Klf4 in the Mouse Cornea
Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3360 - 3370.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. K. Swamynathan, J. P. Katz, K. H. Kaestner, R. Ashery-Padan, M. A. Crawford, and J. Piatigorsky
Conditional Deletion of the Mouse Klf4 Gene Results in Corneal Epithelial Fragility, Stromal Edema, and Loss of Conjunctival Goblet Cells
Mol. Cell. Biol., January 1, 2007; 27(1): 182 - 194.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
W. Adachi, H. Ulanovsky, Y. Li, B. Norman, J. Davis, and J. Piatigorsky
Serial Analysis of Gene Expression (SAGE) in the Rat Limbal and Central Corneal Epithelium.
Invest. Ophthalmol. Vis. Sci., September 1, 2006; 47(9): 3801 - 3810.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. Adhikary, J. F. Crish, R. Gopalakrishnan, F. Bone, and R. L. Eckert
Involucrin Expression in the Corneal Epithelium: An Essential Role for Sp1 Transcription Factors
Invest. Ophthalmol. Vis. Sci., September 1, 2005; 46(9): 3109 - 3120.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
T. Kawakita, E. M. Espana, H. He, W. Li, C.-Y. Liu, and S. C.G. Tseng
Intrastromal Invasion by Limbal Epithelial Cells Is Mediated by Epithelial-Mesenchymal Transition Activated by Air Exposure
Am. J. Pathol., August 1, 2005; 167(2): 381 - 393.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. E. K. Hutcheon, X. Q. Guo, M. A. Stepp, K. J. Simon, P. H. Weinreb, S. M. Violette, and J. D. Zieske
Effect of Wound Type on Smad 2 and 4 Translocation
Invest. Ophthalmol. Vis. Sci., July 1, 2005; 46(7): 2362 - 2368.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
B. Norman, J. Davis, and J. Piatigorsky
Postnatal Gene Expression in the Normal Mouse Cornea by SAGE
Invest. Ophthalmol. Vis. Sci., February 1, 2004; 45(2): 429 - 440.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. C. Romano, E. M. Espana, S. H. Yoo, M. T. Budak, J. M. Wolosin, and S. C. G. Tseng
Different Cell Sizes in Human Limbal and Central Corneal Basal Epithelia Measured by Confocal Microscopy and Flow Cytometry
Invest. Ophthalmol. Vis. Sci., December 1, 2003; 44(12): 5125 - 5129.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
P. M. Ladage, D. H. Ren, W. M. Petroll, J. V. Jester, J. P. G. Bergmanson, and H. D. Cavanagh
Effects of Eyelid Closure and Disposable and Silicone Hydrogel Extended Contact Lens Wear on Rabbit Corneal Epithelial Proliferation
Invest. Ophthalmol. Vis. Sci., May 1, 2003; 44(5): 1843 - 1849.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
T. Nagasaki and J. Zhao
Centripetal Movement of Corneal Epithelial Cells in the Normal Adult Mouse
Invest. Ophthalmol. Vis. Sci., February 1, 2003; 44(2): 558 - 566.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
M. Grueterich and S. C. G. Tseng
Human Limbal Progenitor Cells Expanded on Intact Amniotic Membrane Ex Vivo
Arch Ophthalmol, June 1, 2002; 120(6): 783 - 790.
[Abstract] [Full Text] [PDF]

Home page
 Br J OphthalmolHome page
D Meller, R T F Pires, and S C G Tseng
Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures
Br J Ophthalmol, April 1, 2002; 86(4): 463 - 471.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
P. M. Ladage, K. Yamamoto, D. H. Ren, L. Li, J. V. Jester, W. M. Petroll, J. P. G. Bergmanson, and H. D. Cavanagh
Proliferation Rate of Rabbit Corneal Epithelium during Overnight Rigid Contact Lens Wear
Invest. Ophthalmol. Vis. Sci., November 1, 2001; 42(12): 2804 - 2812.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
C. M. Francesconi, A. E. K. Hutcheon, E.-H. Chung, A. C. Dalbone, N. C. Joyce, and J. D. Zieske
Expression Patterns of Retinoblastoma and E2F Family Proteins during Corneal Development
Invest. Ophthalmol. Vis. Sci., April 1, 2000; 41(5): 1054 - 1062.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
D. D. S. Iglesia, P. H. Gala, T. Qiu, and M. A. Stepp
Integrin Expression During Epithelial Migration and Restratification in the Tenascin-C-deficient Mouse Cornea
J. Histochem. Cytochem., March 1, 2000; 48(3): 363 - 376.
[Abstract] [Full Text]

Home page
 Arch OphthalmolHome page
N. Dushku, S. L. S. Hatcher, D. M. Albert, and T. W. Reid
p53 Expression and Relation to Human Papillomavirus Infection in Pingueculae, Pterygia, and Limbal Tumors
Arch Ophthalmol, December 1, 1999; 117(12): 1593 - 1599.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
E.-H. Chung, A. E. K. Hutcheon, N. C. Joyce, and J. D. Zieske
Synchronization of the G1/S Transition in Response to Corneal Debridement
Invest. Ophthalmol. Vis. Sci., August 1, 1999; 40(9): 1952 - 1958.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Sun, T.-T. Sun, and R. M. Lavker
Identification of a Cytosolic NADP+-dependent Isocitrate Dehydrogenase That Is Preferentially Expressed in Bovine Corneal Epithelium. A CORNEAL EPITHELIAL CRYSTALLIN
J. Biol. Chem., June 11, 1999; 274(24): 17334 - 17341.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
G. Pellegrini, O. Golisano, P. Paterna, A. Lambiase, S. Bonini, P. Rama, and M. De Luca
Location and Clonal Analysis of Stem Cells and Their Differentiated Progeny in the Human Ocular Surface
J. Cell Biol., May 17, 1999; 145(4): 769 - 782.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Y Danjo and I. Gipson
Actin 'purse string' filaments are anchored by E-cadherin-mediated adherens junctions at the leading edge of the epithelial wound, providing coordinated cell movement
J. Cell Sci., January 11, 1998; 111(22): 3323 - 3332.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
M. Lehrer, T. Sun, and R. Lavker
Strategies of epithelial repair: modulation of stem cell and transit amplifying cell proliferation
J. Cell Sci., January 10, 1998; 111(19): 2867 - 2875.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
R. Wu, S Galvin, S. Wu, C Xu, M Blumenberg, and T. Sun
A 300 bp 5'-upstream sequence of a differentiation-dependent rabbit K3 keratin gene can serve as a keratinocyte-specific promoter
J. Cell Sci., January 6, 1993; 105(2): 303 - 316.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Cao, Z. Zhao, R. Mohan, J. Alroy, P. Stanley, and N. Panjwani
Role of the Lewisx Glycan Determinant in Corneal Epithelial Cell Adhesion and Differentiation
J. Biol. Chem., June 8, 2001; 276(24): 21714 - 21723.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1991 by the Association for Research in Vision and Ophthalmology