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Investigative Ophthalmology & Visual Science, Vol 37, 436-443, Copyright © 1996 by Association for Research in Vision and Ophthalmology
ARTICLES AND REPORTS |
A Khaliq, J Jarvis-Evans, D McLeod and M Boulton
Department of Ophthalmology, University of Manchester, England.
PURPOSE. To determine if low oxygen affects growth factor responsiveness in retinal pigment epithelial (RPE) cells and if such effects are mediated through changes in cell surface receptors. METHODS. Proliferating human RPE cells were exposed to varying concentrations of exogenous basic fibroblast growth factor (bFGF) or epidermal growth factor (EGF) at different media oxygen tensions (16 to 147 mm Hg) and cell counts determined after 4 days. Receptor expression was determined by affinity cross-linking and saturation binding studies on confluent RPE cultures exposed to varying media oxygen tensions for 2 days. RESULTS. Retinal pigment epithelial cells exhibited a greater proliferative response to exogenous growth factors at hypoxia than at higher media oxygen tensions, and they expressed bFGF and demonstrated that hypoxia caused both an increase in the number of EGF receptors per cell and a shift from low to high affinity receptors. CONCLUSIONS. These results suggest that hypoxia not only can stimulate RPE cell proliferation per se, it also can "prime" cells t respond more markedly to exogenous growth factors. These observations may be important in elucidating the cause of proliferative vitreoretinopathy.
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