| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Investigative Ophthalmology & Visual Science, Vol 40, 197-202, Copyright © 1999 by Association for Research in Vision and Ophthalmology
ARTICLES AND REPORTS |
H Matsunaga, JT Handa, A Aotaki-Keen, SW Sherwood, MD West and LM Hjelmeland
Department of Ophthalmology, University of California Davis, USA.
PURPOSE: To investigate the relation of senescence-related beta- galactosidase activity and telomere shortening to replicative senescence in cultured human retinal pigment epithelial (RPE) cells. METHODS: A human RPE cell line was serially passaged until 80% of cells were nondividing in a 72-hour 5-bromo-2'-deoxyuridine (BrdU) labeling study. Early- and late-passage cells were double-stained for BrdU and senescence-related beta-galactosidase activity (pH 6). The average chromosomal telomere length at several population doublings was estimated by Southern blot analysis after double digestion of DNA with RsaI and HinfI and using a telomere-specific probe. RESULTS: BrdU-beta- galactosidase double-staining revealed an inverse correlation between the number of BrdU-labeled nuclei and beta-galactosidase-labeled cells as a function of population doubling level (PDL). At PDL 58, only 20% of all cells labeled for BrdU, whereas 57% stained for beta- galactosidase. The mean terminal restriction fragment length (TRF) was reduced from 10 kb in early (PDL 12) cultures to 4 kb in late (PDL 57) cultures. CONCLUSIONS: Senescence-related beta-galactosidase activity and mean TRF length may prove useful in studying the senescence of RPE cells in vitro. These techniques may be valuable in determining senescence of the retinal pigment epithelium in vivo, where senescent RPE cells could be involved in the development of age-related maculopathy and age-related macular degeneration.
This article has been cited by other articles:
|
|
 |
|
  D. Zhu, J. Wu, C. Spee, S. J. Ryan, and D. R. Hinton BMP4 Mediates Oxidative Stress-induced Retinal Pigment Epithelial Cell Senescence and Is Overexpressed in Age-related Macular Degeneration J. Biol. Chem., April 3, 2009; 284(14): 9529 - 9539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Yu, R. Fuchshofer, D. Kook, A. Kampik, H. Bloemendal, and U. Welge-Lussen Subtoxic Oxidative Stress Induces Senescence in Retinal Pigment Epithelial Cells via TGF-{beta} Release Invest. Ophthalmol. Vis. Sci., February 1, 2009; 50(2): 926 - 935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yamazaki, H. Matsunaga, M. Nishikawa, A. Ando, S. Kaneko, K. Okuda, M. Wada, S. Ito, and M. Matsumura Senescence in cultured trabecular meshwork cells Br J Ophthalmol, June 1, 2007; 91(6): 808 - 811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ruiz-Torres, R. Lozano, J. Melon, and R. Carraro Age-Dependent Decline of In Vitro Migration (Basal and Stimulated by IGF-1 or Insulin) of Human Vascular Smooth Muscle Cells J. Gerontol. A Biol. Sci. Med. Sci., December 1, 2003; 58(12): B1074 - 1077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Guirao, M. Redondo, E. Geraghty, P. Piers, S. Norrby, and P. Artal Corneal Optical Aberrations and Retinal Image Quality in Patients in Whom Monofocal Intraocular Lenses Were Implanted Arch Ophthalmol, September 1, 2002; 120(9): 1143 - 1151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Rambhatla, C.-P. Chiu, R. D. Glickman, and C. Rowe-Rendleman In Vitro Differentiation Capacity of Telomerase Immortalized Human RPE Cells Invest. Ophthalmol. Vis. Sci., May 1, 2002; 43(5): 1622 - 1630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Honda, L. M Hjelmeland, and J. T Handa Senescence associated {beta} galactosidase activity in human retinal pigment epithelial cells exposed to mild hyperoxia in vitro Br J Ophthalmol, February 1, 2002; 86(2): 159 - 162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Honda, L. M. Hjelmeland, and J. T. Handa Oxidative Stress-Induced Single-Strand Breaks in Chromosomal Telomeres of Human Retinal Pigment Epithelial Cells In Vitro Invest. Ophthalmol. Vis. Sci., August 1, 2001; 42(9): 2139 - 2144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Miyamura, K. Mishima, S. Honda, A. E. Aotaki-Keen, L. S. Morse, J. T. Handa, and L. M. Hjelmeland Age and Topographic Variation of Insulin-like Growth Factor-Binding Protein 2 in the Human RPE Invest. Ophthalmol. Vis. Sci., June 1, 2001; 42(7): 1626 - 1630. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Ueda, K. K. Wentz–Hunter, E. L. Cheng, T. Fukuchi, H. Abe, and B. Y.J.T. Yue Ultrastructural Localization of Myocilin in Human Trabecular Meshwork Cells and Tissues J. Histochem. Cytochem., October 1, 2000; 48(10): 1321 - 1330. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Kurz, S Decary, Y Hong, and J. Erusalimsky Senescence-associated (beta)-galactosidase reflects an increase in lysosomal mass during replicative ageing of human endothelial cells J. Cell Sci., January 10, 2000; 113(20): 3613 - 3622. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
