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(Investigative Ophthalmology and Visual Science. 1999;40:2235-2241.)
© 1999 by The Association for Research in Vision and Ophthalmology, Inc.

{alpha}B-Crystallin in the Trabecular Meshwork Is Inducible by Transforming Growth Factor-ß

Ulrich Welge–Lüßen1, Christian Albrecht May1, Michael Eichhorn1, Hans Bloemendal2 and Elke Lütjen–Drecoll1

1 From the Department of Anatomy II, University of Erlangen–Nürnberg, Erlangen, Germany; and the 2 Department of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands.

Abstract

PURPOSE. Because in glaucomatous eyes transforming growth factor-ß (TGF-ß) and {alpha}B-crystallin are increased in the anterior eye segment, the effect of TGF-ß1 and TGF-ß2 on the expression of {alpha}B-crystallin and its corresponding mRNA was studied in human trabecular meshwork (TM) cells.

METHODS. Monolayer cultures of "cribriform" and "corneoscleral" TM cells of 5 human donors (12–73 years of age) were treated with either 1.0 ng/ml TGF-ß1, TGF-ß2, or 5 x 10-7 dexamethasone (DEX) for 12 to 96 hours. Induction of {alpha}B-crystallin and the related mRNA was investigated by western and northern blot analyses. For comparison, human foreskin fibroblasts (HFF) and NIH 3T3 cells were treated in the same way as the TM cells.

RESULTS. An increase of {alpha}B-crystallin mRNA was observed after treatment of TM cells with TGF-ß1 and TGF-ß2, whereas DEX had no effect. In the cribriform TM cells with a high basal level, the enhancement ranged between 2 and 3 times; whereas in the corneoscleral TM cells {alpha}B-crystallin mRNA increased between 5 and 6 times. Using western blot analysis, the increase of {alpha}B-crystallin expression in the cribriform TM cells was only small compared with the significant increase in the corneoscleral TM cells. Treatment of HFF and NIH 3T3 cells with TGF-ß did not induce {alpha}B-crystallin mRNA.

CONCLUSIONS. This is the first time to show that {alpha}B-crystallin is not only induced by stress factors but also by TGF-ß in TM cell cultures. The difference in induction of mRNA and protein seems to be dependent on {alpha}B-crystallin concentration before treatment.




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