| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 From the The Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School; and the 2 Department of Oral Medicine and Diagnostic Sciences, Harvard School of Dental Medicine, Boston.
Abstract
PURPOSE. To demonstrate the specific binding of autoantibodies present in the sera of patients with ocular cicatricial pemphigoid (OCP) to human ß4 integrin present in the normal human conjunctiva (NHC) and to study the role of OCP autoantibodies and antibody to human ß4 integrin in the pathogenesis of subepithelial lesion formation in OCP.
METHODS. Indirect immunofluorescence assay and in vitro organ culture method
using NHC were used. Sera and IgG fractions from 10 patients with OCP;
immunoaffinity-purified OCP autoantibody; antibodies to human ß4,
ß1, 
6, and 5 integrins; and sera from patients with pemphigus
vulgaris, bullous pemphigoid (BP), and chronic atopic and chronic
ocular rosacea cicatrizing conjunctivitis; and normal human serum (NHS)
were used.
RESULTS. Nine of 10 OCP sera or IgG fractions, immunoaffinity-purified OCP
autoantibody, antibodies to human ß4 and 6 integrins, and sera
from patients with BP showed homogenous, smooth linear binding along
the basement membrane zone (BMZ) of the NHC. NHS, antibodies to other
integrins, and sera from patients with chronic cicatrizing
conjunctivitis from other causes showed no such binding. When NHC was
first absorbed with OCP sera and then reacted with anti-ß4 antibodies
or vice versa, the intensity of the BMZ binding was dramatically
reduced or completely eliminated, indicating that there were
autoantibodies in OCP sera specific for the ß4 integrin. BMZ
separation developed 48 to 72 hours after addition of total OCP sera,
IgG fractions from OCP sera, immunoaffinity-purified autoantibodies
from sera of patients with OCP, or anti-ß4 antibodies to the NHC
cultures, but not after addition of normal control sera, sera from
patients with chronic cicatrizing conjunctivitis from causes other than
OCP, or sera from patients with OCP in clinical remission.
CONCLUSION. Circulating anti-ß4 integrin antibody may have an important role in the pathogenesis of OCP.
This article has been cited by other articles:
|
|
 |
|
  J Velozo, S Aguilera, C Alliende, P Ewert, C Molina, P Perez, L Leyton, A Quest, M Brito, S Gonzalez, et al. Severe alterations in expression and localisation of {alpha}6{beta}4 integrin in salivary gland acini from patients with Sjogren syndrome Ann Rheum Dis, June 1, 2009; 68(6): 991 - 996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Dainichi, S. Kurono, B. Ohyama, N. Ishii, N. Sanzen, M. Hayashi, C. Shimono, Y. Taniguchi, H. Koga, T. Karashima, et al. Anti-laminin gamma-1 pemphigoid PNAS, February 24, 2009; 106(8): 2800 - 2805. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Letko, K. Bhol, F. Anzaar, V. L. Perez, A. R. Ahmed, and C. S. Foster Chronic Cicatrizing Conjunctivitis in a Patient With Epidermolysis Bullosa Acquisita. Arch Ophthalmol, November 1, 2006; 124(11): 1615 - 1618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Sitaru, A. Kromminga, T. Hashimoto, E. B. Brocker, and D. Zillikens Autoantibodies to Type VII Collagen Mediate Fc{gamma}-Dependent Neutrophil Activation and Induce Dermal-Epidermal Separation in Cryosections of Human Skin Am. J. Pathol., July 1, 2002; 161(1): 301 - 311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S. Chan, A. R. Ahmed, G. J. Anhalt, W. Bernauer, K. D. Cooper, M. J. Elder, J.-D. Fine, C. S. Foster, R. Ghohestani, T. Hashimoto, et al. The First International Consensus on Mucous Membrane Pemphigoid: Definition, Diagnostic Criteria, Pathogenic Factors, Medical Treatment, and Prognostic Indicators Arch Dermatol, March 1, 2002; 138(3): 370 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.C. Bhol, L. Goss, S. Kumari, J.E. Colon, and A.R. Ahmed Autoantibodies to Human {alpha}6 Integrin in Patients with Oral Pemphigoid Journal of Dental Research, August 1, 2001; 80(8): 1711 - 1715. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Kumari, K. C. Bhol, R. K. Simmons, M. S. Razzaque, E. Letko, C. S. Foster, and A. R. Ahmed Identification of Ocular Cicatricial Pemphigoid Antibody Binding Site(s) in Human {beta}4 Integrin Invest. Ophthalmol. Vis. Sci., February 1, 2001; 42(2): 379 - 385. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. C. Bhol, M. J. Dans, R. K. Simmons, C. S. Foster, F. G. Giancotti, and A. R. Ahmed The Autoantibodies to {alpha}6{beta}4 Integrin of Patients Affected by Ocular Cicatricial Pemphigoid Recognize Predominantly Epitopes Within the Large Cytoplasmic Domain of Human {beta}4 J. Immunol., September 1, 2000; 165(5): 2824 - 2829. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
