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Enhanced Secretory Group II PLA₂ Activity in the Tears of Chronic Blepharitis Patients

¹ From the Department of Ophthalmology, Chung–Ang University Hospital and ² Department of Environmental and Health Chemistry, College of Pharmacy, Chung–Ang University, Seoul, Korea.

PURPOSE. Phospholipase A₂ (PLA₂) hydrolyzes phospholipids, one of the important constituents of human meibomian gland secretions. This study was performed to investigate PLA₂ type and activity in the tears of chronic blepharitis patients compared to those of normal persons.

METHODS. Tear samples of 36 patients and 10 normal persons were collected in non-heparinized microcapillary tubes. PLA₂ activity in the tears was measured by Dole’s method, and the results of the blepharitis patients were compared to those of the normal persons. The characterization of PLA₂ was performed by the head group preference test and the dithiothreitol (DTT) sensitivity test. The classification of PLA₂ type was done using Western blot analysis with anti-human secretory PLA₂ antibody.

RESULTS. No statistically significant differences were found among the six categories of chronic blepharitis. However, the mean PLA₂ activity in the tears of the chronic blepharitis patients was about two times higher than that of the normal controls with statistical significance (P < 0.05). The PLA₂ substrate specificity test revealed group II PLA₂ activity. Furthermore, the group II PLA₂ was identified as a 14 kDa band in Western blot analysis using an antibody raised against human secretory group II PLA₂.

CONCLUSIONS. Secretory group II PLA₂ activity was significantly enhanced in the tears of the chronic blepharitis patients compared with that of the normal controls. It is suggested that this increased enzymatic activity may decrease the tear film stability through increased hydrolysis of phospholipids.

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