IOVS AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wenkel, H.
Right arrow Articles by Milton, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wenkel, H.
Right arrow Articles by Milton, J.
(Investigative Ophthalmology and Visual Science. 1999;40:3058-3062.)
© 1999 by The Association for Research in Vision and Ophthalmology, Inc.

Modulation of Retinal Pigment Epithelial Cell Behavior by Agaricus Bisporus Lectin

Hartmut Wenkel1,2,3, David Kent1, Paul Hiscott1, Mark Batterbury1, Carl Groenewald1, Carl M. Sheridan1, Lu–Gang Yu4 and Jeremy Milton4

1 From the Unit of Ophthalmology, Department of Medicine and St. Paul’s Eye Unit, University of Liverpool, UK; 2 The Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts; the 3 Department of Ophthalmology, University of Erlangen–Nürnberg, Erlangen, Germany; and the 4 Unit of Glycobiology, Department of Medicine, University of Liverpool, UK.

PURPOSE. To determine whether Agaricus bisporus lectin (ABL) binds retinal pigment epithelial cells (RPEs), to conduct a preliminary viability study of RPEs exposed to ABL, and to evaluate the effects of ABL on RPE proliferation and RPE-mediated matrix contraction in vitro.

METHODS. Using cultured bovine RPEs, immunohistochemistry was used to study ABL binding. Morphologic and trypan blue exclusion techniques were used for toxicity studies. The effect of ABL on RPE proliferation was investigated by [methyl-3H]–thymidine incorporation. The effect of ABL on RPE-mediated matrix contraction was evaluated with RPE-populated three-dimensional collagen matrices.

RESULTS. ABL bound to RPE cells. This binding was inhibited by asialomucin. No change in RPE morphology or trypan blue exclusion compared with controls was observed in RPEs incubated with 5 to 60 µg/ml ABL for 3 days. Twenty-four-hour incubations of RPEs with ABL significantly inhibited RPE proliferation in a dose-dependent way, 40 µg/ml ABL inhibited proliferation by 83% (SE 14, P < 0.05). ABL showed a dose-dependent significant inhibition of RPE-mediated collagen matrix contraction over 3 days, with 93% inhibition compared with controls by 40 µg/ml lectin (P < 0.05). The inhibitory effect of ABL on proliferation and gel contraction was partly reversible after eliminating ABL from the culture medium.

CONCLUSIONS. Bovine RPE cells bind ABL, and preliminary evaluations suggest that levels of ABL that are nontoxic to the cells potently inhibit RPE proliferation and RPE-mediated matrix contraction. ABL deserves further investigation as a potential inhibitor of RPE proliferation and cell-mediated matrix contraction in anomalous reparative processes such as proliferative vitreoretinopathy and as a laboratory tool for RPE behavioral studies.




This article has been cited by other articles:


Home page
 IOVSHome page
Y. Noda, Y. Hata, T. Hisatomi, Y. Nakamura, K. Hirayama, M. Miura, S. Nakao, K. Fujisawa, T. Sakamoto, and T. Ishibashi
Functional Properties of Hyalocytes under PDGF-Rich Conditions
Invest. Ophthalmol. Vis. Sci., July 1, 2004; 45(7): 2107 - 2114.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the Association for Research in Vision and Ophthalmology