IOVS Genetics
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(Investigative Ophthalmology and Visual Science. 1999;40:3132-3137.)
© 1999 by The Association for Research in Vision and Ophthalmology, Inc.

Photodynamic Tissue Adhesion with Chlorine6 Protein Conjugates

John Khadem1,2, Amadeo A. Veloso, Jr2, Felipe Tolentino2, Tayyaba Hasan3,4 and Michael R. Hamblin3,4

From 1 The Retina Specialists of Boston, Harvard Medical School, 2 Schepens Eye Research Institute; and 3 Wellman Laboratories of Photomedicine, 4 Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Boston.

PURPOSE. To test the hypothesis that a photodynamic laser-activated tissue solder would perform better in sealing scleral incisions when the photosensitizer was covalently linked to the protein than when it was noncovalently mixed.

METHODS. Conjugates and mixtures were prepared between the photosensitizer chlorine6 and various proteins (albumin, fibrinogen, and gelatin) in different ratios and used to weld penetrating scleral incisions made in human cadaveric eyes. A blue-green (488–514 nm) argon laser activated the adhesive, and the strength of the closure was measured by increasing the intraocular pressure until the wound showed leakage.

RESULTS. Both covalent conjugates and noncovalent mixtures showed a light dose-dependent increase in leaking pressure. A preparation of albumin chlorine6 conjugate with additional albumin added (2.5 protein to chlorine6 molar ratio) showed significantly higher weld strength than other protein conjugates and mixtures.

CONCLUSIONS. This is the first report of dye–protein conjugates as tissue solders. These conjugates may have applications in ophthalmology.






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Copyright © 1999 by the Association for Research in Vision and Ophthalmology