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(Investigative Ophthalmology and Visual Science. 1999;40:1812-1818.)
© 1999 by The Association for Research in Vision and Ophthalmology, Inc.

ß-Arrestin–Related Proteins in Ocular Tissues

Corine Nicolas–Léveque, Ibtissem Ghedira, Jean Pierre Faure and Massoud Mirshahi

From the Institut National de la Santé et de la Recherche Médicale, Paris, France.

PURPOSE. Proteins of the arrestin family contribute to the regulation of G-protein–mediated transduction. In this study, the presence of ß-arrestins in ocular tissues was investigated.

METHODS. Mouse monoclonal and rabbit polyclonal antibodies were raised against the peptide Val-Asp-Thr-Asn-Ile-Leu-Glu-Leu-Asp-Thr-Asn-Asp-Asp-Asp-Ile, a sequence present in ß-arrestins 1 and 2 but absent from visual arrestin. These antibodies were used for the immunohistologic detection of ß-arrestins in parafin sections of rodent eyes fixed in Bouin’s solution. Reverse transcription–polymerase chain reaction (RT-PCR) analysis of RNA from bovine retina, retinal pigmented epithelial (RPE) cells, lens epithelial cells, and human corneal fibroblasts was performed using ß-1 arrestin primers.

RESULTS. In the eye, ß-arrestin staining predominated in RPE, inner segments of photoreceptors, synaptic spherules of rods, inner plexiform layer and ganglion cell fibers, epithelial cells from ciliary body, and vessels. RT- PCR amplified a 480 bp product, corresponding to the predicted length. The sequence of PCR products from bovine retina and RPE cells was identical with the bovine ß-arrestin mRNA.

CONCLUSIONS. ß-arrestins were detected in several ocular tissues. In photoreceptor cells, their specific localization in the synaptic terminals and plexiform layer suggests a role of ß-arrestin in synaptic transmission. In other ocular tissues, the presence of ß-arrestin may be related either to adrenergic signal transduction or to signal transduction mediated by other G-protein–coupled receptors.






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Copyright © 1999 by the Association for Research in Vision and Ophthalmology