| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 From the Department of Ophthalmology, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan; and the 2 Department of Physiology and 3 Center of General Education, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan, Republic of China.
PURPOSE. To study the in vitro angiogenic activity of human conjunctival and limbal epithelial cells and conjunctival, limbal, and corneal fibroblasts in a three-cell-type coculture model.
METHODS. Human umbilical vein endothelial cells (EC) were cocultured with epithelial cells, fibroblasts, or epithelial cells and fibroblasts to test their effect on EC morphogenesis. Neutralizing antibodies to some known angiogenic factors were added to the culture to see whether the EC morphogenesis may be blocked by a particular antibody.
RESULTS. Conjunctival and limbal epithelial cells exhibited very little or no stimulatory effect on EC tube formation when examined in an EC–epithelial cell coculture system. In contrast, conjunctival, limbal, and corneal fibroblasts all promoted EC morphogenesis when examined under the same culture conditions. Fibroblast-induced EC morphogenesis was inhibited by addition of anti-vascular endothelial growth factor (VEGF) and/or anti-basic fibroblast growth factor (bFGF) antibodies to the culture medium. In the three-cell-type coculture system consisting of ECs, fibroblasts, and epithelial cells, limbal epithelial cells (but not conjunctival epithelial cells) exhibited a strong inhibitory effect on fibroblast-induced EC tube formation.
CONCLUSIONS. The proangiogenic activity of ocular surface fibroblasts is probably mediated through a paracrine mechanism by VEGF and bFGF. Limbal epithelial cells, but not conjunctival epithelial cells, inhibit fibroblast-stimulated angiogenesis.
This article has been cited by other articles:
|
|
 |
|
  N. Di Girolamo, J. Chui, D. Wakefield, and M. T Coroneo Cultured human ocular surface epithelium on therapeutic contact lenses Br. J. Ophthalmol., April 1, 2007; 91(4): 459 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H.-K. Ma, J.-Y. Yao, M.-T. Kuo, L.-C. See, K.-Y. Lin, S.-C. Chen, J.-K. Chen, A.-S. Chao, S.-F. Wang, and K.-K. Lin Generation of Endostatin by Matrix Metalloproteinase and Cathepsin from Human Limbocorneal Epithelial Cells Cultivated on Amniotic Membrane Invest. Ophthalmol. Vis. Sci., February 1, 2007; 48(2): 644 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H.-K. Ma, J.-Y. Yao, L.-K. Yeh, S.-T. Liang, L.-C. See, H.-T. Chen, K.-Y. Lin, C.-C. Liang, K.-K. Lin, and J.-K. Chen In Vitro Antiangiogenic Activity in Ex Vivo Expanded Human Limbocorneal Epithelial Cells Cultivated on Human Amniotic Membrane Invest. Ophthalmol. Vis. Sci., August 1, 2004; 45(8): 2586 - 2595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Leonardi, P. Brun, M. Tavolato, G. Abatangelo, M. Plebani, and A. G. Secchi Growth Factors and Collagen Distribution in Vernal Keratoconjunctivitis Invest. Ophthalmol. Vis. Sci., December 1, 2000; 41(13): 4175 - 4181. [Abstract] [Full Text]
|
|
|
|
|
|
D. H.-K. Ma, L.-C. See, S.-B. Liau, and R. J.-F. Tsai Amniotic membrane graft for primary pterygium: comparison with conjunctival autograft and topical mitomycin C treatment Br. J. Ophthalmol., September 1, 2000; 84(9): 973 - 978. [Abstract] [Full Text]
|
|
|
|
|
|
W. Philipp, L. Speicher, and C. Humpel Expression of Vascular Endothelial Growth Factor and Its Receptors in Inflamed and Vascularized Human Corneas Invest. Ophthalmol. Vis. Sci., August 1, 2000; 41(9): 2514 - 2522. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
