| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 From the St. Paul’s Eye Unit, Royal Liverpool University Hospital; and the 2 Unit of Ophthalmology, Department of Medicine, and 3 Department of Clinical Engineering, University of Liverpool, United Kingdom.
PURPOSE. Hepatocyte growth factor/scatter factor (HGF/SF) possesses mitogenic, motogenic, and morphogenic properties and has recently been implicated in various retinal diseases. The role of HGF/SF in proliferative vitreoretinal disease was investigated.
METHODS. Sections of epiretinal membranes were stained immunohistochemically for cytokeratins, to identify HRPE cells, and for HGF/SF receptor (c-Met). Cultured HRPE cells were stained for c-Met and investigated for shape change in response to HGF/SF, by using image analysis. The dose–response relationship for HRPE cells to HGF/SF was investigated by a cell migration assay and the specificity of this response evaluated by a neutralization experiment. Subretinal fluid (SRF) and vitreous from patients with retinal detachment and proliferative vitreoretinopathy (PVR) plus vitreous from eyes obtained after death, eyes with macular hole, and eyes with proliferative diabetic retinopathy (PDR) were investigated for the presence of HGF/SF using an enzyme-linked immunosorbent assay (ELISA). HGF/SF activity was measured using an MDCK cell scatter assay.
RESULTS. HRPE cells in epiretinal membranes and in culture expressed c-Met. Cultured HRPE cells responded to HGF/SF by an epithelial-to-mesenchymal shape change and by cell migration, a response that increased with increasing concentrations of HGF/SF. This response was reduced in the presence of neutralizing antibody. There was evidence of HGF/SF in increasing concentrations in more severe PVR and in PDR when measured by ELISA, and, conversely, there was evidence of correspondingly decreasing HGF/SF activity when measured by MDCK cell scatter assay in these diseases.
CONCLUSIONS. HGF/SF is present in normal and pathologic vitreous. HRPE cells respond by shape change and cell migration to HGF/SF. Concentrations of HGF/SF increase in proliferative vitreoretinal disease and increase in turn with increased severity of the disease, but HGF/SF bioactivity decreases (consistent with activator depletion). These findings are consistent with the hypothesis that HGF/SF may play a role in the HRPE mesenchymal transformation that typifies PVR.
This article has been cited by other articles:
|
|
 |
|
  H.-J. Chen and Z.-Z. Ma N-Cadherin Expression in a Rat Model of Retinal Detachment and Reattachment Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1832 - 1838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Alge, S. G. Priglinger, D. Kook, H. Schmid, C. Haritoglou, U. Welge-Lussen, and A. Kampik Galectin-1 Influences Migration of Retinal Pigment Epithelial Cells Invest. Ophthalmol. Vis. Sci., January 1, 2006; 47(1): 415 - 426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Dieudonne, E. C. La Heij, R. Diederen, A. G. H. Kessels, A. T. A. Liem, A. Kijlstra, and F. Hendrikse High TGF-{beta}2 Levels during Primary Retinal Detachment May Protect against Proliferative Vitreoretinopathy Invest. Ophthalmol. Vis. Sci., November 1, 2004; 45(11): 4113 - 4118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jin, Y. Chen, S. He, S. J. Ryan, and D. R. Hinton Hepatocyte Growth Factor and its Role in the Pathogenesis of Retinal Detachment Invest. Ophthalmol. Vis. Sci., January 1, 2004; 45(1): 323 - 329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Troger, S Sellemond, G Kieselbach, M Kralinger, E Schmid, B Teuchner, Q A Nguyen, E Schretter-Irschick, and W Gottinger Inhibitory effect of certain neuropeptides on the proliferation of human retinal pigment epithelial cells Br. J. Ophthalmol., November 1, 2003; 87(11): 1403 - 1408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. Van Aken, O. De Wever, L. Van Hoorde, E. Bruyneel, J.-J. De Laey, and M. M. Mareel Invasion of Retinal Pigment Epithelial Cells: N-cadherin, Hepatocyte Growth Factor, and Focal Adhesion Kinase Invest. Ophthalmol. Vis. Sci., February 1, 2003; 44(2): 463 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Hogg, I. Grierson, and P. Hiscott Direct Comparison of the Migration of Three Cell Types Involved in Epiretinal Membrane Formation Invest. Ophthalmol. Vis. Sci., August 1, 2002; 43(8): 2749 - 2757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ikuno and A. Kazlauskas An In Vivo Gene Therapy Approach for Experimental Proliferative Vitreoretinopathy Using the Truncated Platelet-Derived Growth Factor {alpha} Receptor Invest. Ophthalmol. Vis. Sci., July 1, 2002; 43(7): 2406 - 2411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ikuno, F.-L. Leong, and A. Kazlauskas PI3K and PLC{gamma} Play a Central Role in Experimental PVR Invest. Ophthalmol. Vis. Sci., February 1, 2002; 43(2): 483 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ikuno and A. Kazlauskas TGF{beta}1-Dependent Contraction of Fibroblasts Is Mediated by the PDGF{alpha} Receptor Invest. Ophthalmol. Vis. Sci., January 1, 2002; 43(1): 41 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Wajih, J. Walter, and D. C. Sane Vascular Origin of a Soluble Truncated Form of the Hepatocyte Growth Factor Receptor (c-met) Circ. Res., January 11, 2002; 90(1): 46 - 52. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
