HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mulroy, L. Articles by Kochevar, I. E. Search for Related Content PubMed PubMed Citation Articles by Mulroy, L. Articles by Kochevar, I. E.

Photochemical Keratodesmos for Repair of Lamellar Corneal Incisions

¹ From the Massachusetts General Hospital, Wellman Laboratories of Photomedicine, Department of Dermatology, and ² Cornea and Refractive Services, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston.

PURPOSE. To determine the efficacy of photochemical keratodesmos (PKD) for closing surgical incisions in the cornea of enucleated rabbit eyes compared with that achieved using sutures and self-sealing incisions.

METHODS. A 3.5-mm incision, at an angle parallel to the iris, was made in the cornea of enucleated New Zealand White rabbit eyes. The intraocular pressure required to cause leakage (IOP_L) from the untreated incision was then recorded. Photochemical keratodesmos treatment was then performed by application of a dye, Rose Bengal (RB), in saline solution to the surfaces of the incision wound, followed by laser irradiation at 514 nm from an argon ion laser. Immediately after treatment, the IOP_L was measured. Both dose and laser irradiance dependencies were studied in five or more eyes for each condition and appropriate control eyes. The IOP_Ls were compared with those obtained using conventional interrupted 10-0 nylon sutures. Other dyes were tested in a similar fashion.

RESULTS. The IOP_L of 300 mm Hg was obtained using a fluence of 1270 J/cm² with an irradiance of 1.27 W/cm² (laser exposure time, 16 minutes 40 seconds). No sealing was observed using dye or light alone where control pressures of approximately 30 mm Hg were found. At higher dose (1524 J/cm²) and irradiance (3.82 W/cm²; 6 minutes 35 seconds), PKD was less effective, which may be attributable to thermal effects. PKD produced IOP_Ls similar to those in closure by sutures. Other dyes such as riboflavin-5-phosphate and N-hydroxy-pyridine thione also produced efficient bonding after PKD. Nonphotochemically active dyes did not produce significant increases in the IOP_L at which leakage occurred.

CONCLUSIONS. The increase in IOP_L after PKD treatment, comparable with that with sutures, in enucleated rabbit eyes demonstrates the feasibility of this technique ex vivo.

This article has been cited by other articles:

				J. M. G. Reyes, S. Herretes, A. Pirouzmanesh, D.-A. Wang, J. H. Elisseeff, A. Jun, P. J. McDonnell, R. S. Chuck, and A. Behrens A Modified Chondroitin Sulfate Aldehyde Adhesive for Sealing Corneal Incisions Invest. Ophthalmol. Vis. Sci., April 1, 2005; 46(4): 1247 - 1250. [Abstract] [Full Text] [PDF]

				C. E. Proano, L. Mulroy, E. Jones, D. T. Azar, R. W. Redmond, and I. E. Kochevar Photochemical Keratodesmos for Bonding Corneal Incisions Invest. Ophthalmol. Vis. Sci., July 1, 2004; 45(7): 2177 - 2181. [Abstract] [Full Text] [PDF]