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(Investigative Ophthalmology and Visual Science. 2000;41:3732-3742.)
© 2000 by The Association for Research in Vision and Ophthalmology, Inc.

Androgen Influence on the Meibomian Gland

David A. Sullivan1, Benjamin D. Sullivan1, M. David Ullman2, Eduardo M. Rocha1, Kathleen L. Krenzer1,3, Jennifer M. Cermak1, Ikuko Toda1, Marshall G. Doane1, James E. Evans4 and L. Alexandra Wickham1

1 From the Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical School, Boston; 2 Edith Norse Rogers Veterans Memorial Hospital, Bedford; the 3 New England College of Optometry, Boston; and the 4 Eunice Kennedy Shriver Center for Mental Retardation, Waltham, Massachusetts.

PURPOSE. The hypothesis in the study was that androgens control meibomian gland function, regulate the quality and/or quantity of lipids produced by this tissue, and promote the formation of the tear film’s lipid layer. To test this hypothesis, a study was conducted to determine whether androgen receptor protein exists in the epithelial cell nuclei of rat meibomian glands and, in addition, whether androgen deficiency and/or treatment influences the gross morphology, neutral lipid content, and fatty acid profile of the rabbit meibomian gland, as well as the appearance of the tear film lipid layer.

METHODS. Rat lids were obtained and processed for immunohistochemistry. Meibomian glands from intact, androgen- and/or placebo-treated rabbits were analyzed by histology, and glandular lipids were evaluated by gas chromatography, high-performance liquid chromatography (HPLC), and mass spectrometry. The rabbit tear film lipid layer was assessed by interferometry.

RESULTS. In the current study androgen receptor protein existed within acinar epithelial cell nuclei of rat meibomian glands; androgen deficiency was associated with alterations in the lipid content of the rabbit meibomian gland; 19-nortestosterone treatment modulated the fatty acid profile in the total and neutral lipid fractions of the rabbit meibomian gland; and androgens did not appear to influence the gross morphology of meibomian tissue or to exert a demonstrable effect on the rabbit tear film lipid layer.

CONCLUSIONS. The findings show that the meibomian gland is an androgen target organ and that androgens influence the lipid profile within this tissue. However, the extent to which androgens regulate the production of these lipids and whether this action may impact tear film stability remain to be determined.




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