HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kaifi, J. T. Articles by Pearlman, E. Search for Related Content PubMed PubMed Citation Articles by Kaifi, J. T. Articles by Pearlman, E.

Impaired Eosinophil Recruitment to the Cornea in P-Selectin–Deficient Mice in Onchocerca volvulus Keratitis (River Blindness)

¹ From the Departments of Medicine, Ophthalmology, and Pathology, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, Ohio; and ² Genetics Institute/Wyeth Ayerst Research, Andover, Massachusetts.

PURPOSE. A murine model of helminth-induced keratitis (river blindness) that is characterized by a biphasic recruitment of neutrophils (days 1–3) and eosinophils (days 3+) to the cornea has been developed. The purpose of this study was to determine the relative contribution of P- and E-selectin in recruitment of these inflammatory cells from limbal vessels to the corneal stroma.

METHODS. P- and E-selectin gene knockout (-/-) mice were immunized with antigens extracted from the parasitic helminth Onchocerca volvulus. One week after the last immunization, parasite antigens were injected directly into the corneal stroma. Mice were killed on days 1 and 3 postchallenge, and eyes were immunostained with either anti-eosinophil major basic protein (MBP) or with anti-neutrophil Ab. The number of cells in the cornea was determined by direct counting.

RESULTS. Recruitment of eosinophils to the cornea was significantly impaired in P-selectin^-/- mice (63.9% fewer eosinophils on day 1 [P = 0.0015], and 61% fewer on day 3 [P < 0.0001]) compared with control C57BL/6 mice. In contrast, P-selectin deficiency had no effect on neutrophil recruitment to the cornea. There was no inhibition of eosinophil and neutrophil migration to the corneas of E-selectin^-/- mice, indicating that there is no direct role for this adhesion molecule in helminth-induced keratitis.

CONCLUSIONS. The present study demonstrates that P-selectin is an important mediator of eosinophil recruitment to the cornea. P-selectin interactions may therefore be potential targets for immunotherapy in eosinophil-mediated ocular inflammation.

This article has been cited by other articles:

				R. B. Berger, N. M. Blackwell, J. H. Lass, E. Diaconu, and E. Pearlman IL-4 and IL-13 Regulation of ICAM-1 Expression and Eosinophil Recruitment in Onchocerca volvulus Keratitis Invest. Ophthalmol. Vis. Sci., September 1, 2002; 43(9): 2992 - 2997. [Abstract] [Full Text] [PDF]

				L. R. Hall, E. Diaconu, and E. Pearlman A Dominant Role for Fc{{gamma}} Receptors in Antibody-Dependent Corneal Inflammation J. Immunol., July 15, 2001; 167(2): 919 - 925. [Abstract] [Full Text] [PDF]

				J. T. Kaifi, E. Diaconu, and E. Pearlman Distinct Roles for PECAM-1, ICAM-1, and VCAM-1 in Recruitment of Neutrophils and Eosinophils to the Cornea in Ocular Onchocerciasis (River Blindness) J. Immunol., June 1, 2001; 166(11): 6795 - 6801. [Abstract] [Full Text] [PDF]