IOVS The FASEB Journal
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ueda, H.
Right arrow Articles by Ohno, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ueda, H.
Right arrow Articles by Ohno, S.
(Investigative Ophthalmology and Visual Science. 2000;41:3908-3914.)
© 2000 by The Association for Research in Vision and Ophthalmology, Inc.

Dystrobrevin Localization in Photoreceptor Axon Terminals and at Blood–Ocular Barrier Sites

Hideho Ueda1, Takeshi Baba1, Kenji Kashiwagi2, Hiroyuki Iijima2 and Shinichi Ohno1

1 From the Departments of Anatomy and 2 Ophthalmology, Yamanashi Medical University, Japan.

PURPOSE. Dystrobrevin is a newly discovered dystrophin-associated protein with multiple sites for phosphorylation on tyrosine residues. In the present study, the cellular distribution and subcellular localization of dystrobrevin were examined in the adult rat retina, cornea, lens, iris, ciliary body, and cultured Müller cells.

METHODS. Immunoblot analysis, confocal laser scanning microscopy, and immunoelectron microscopy were used to examine dystrobrevin expression.

RESULTS. Immunoblot analysis showed that an approximately 87-kDa band was expressed predominantly in the lens, retina, iris and ciliary body, whereas an approximately 60-kDa band was expressed in cultured Müller cells, cornea, retina, iris, and ciliary body. Confocal microscopy demonstrated dystrobrevin in the inner limiting membrane, outer plexiform layer, and retinal pigment epithelium and around blood vessels in the retina. At the ultrastructural level, dystrobrevin was localized under cell membranes of rod spherules and cone pedicles of photoreceptor cell terminals but often was found in the cytoplasm of endothelial cells and Müller cells. Furthermore, dystrobrevin was colocalized with ß-dystroglycan in corneal endothelium; lens, iris, and ciliary epithelia; and cultured Müller cells.

CONCLUSIONS. The present study demonstrates that dystrobrevin is expressed in neurons, glia, and endothelial cells in the rat retina. In addition, dystrobrevin is localized at the blood–ocular barrier sites in extraocular tissue. These data suggest that dystrobrevin plays an important role in visual function.




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
R. M. Grady, D. F. Wozniak, K. K. Ohlemiller, and J. R. Sanes
Cerebellar synaptic defects and abnormal motor behavior in mice lacking alpha- and beta-dystrobrevin.
J. Neurosci., March 15, 2006; 26(11): 2841 - 2851.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Association for Research in Vision and Ophthalmology