IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ruether, K.
Right arrow Articles by Hartmann, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ruether, K.
Right arrow Articles by Hartmann, C.
(Investigative Ophthalmology and Visual Science. 2000;41:4039-4047.)
© 2000 by The Association for Research in Vision and Ophthalmology, Inc.

Abnormalities of the Photoreceptor-Bipolar Cell Synapse in a Substrain of C57BL/10 Mice

Klaus Ruether1, Johannes Grosse2, Eike Matthiessen3, Kirstin Hoffmann1 and Christian Hartmann3

1 From the UKE Eye Hospital, University of Hamburg, Germany; 2 Institute of Anatomy, Technical University, München, Germany; and 3 Charité-Virchow Eye Hospital, Humboldt University, Berlin, Germany.

PURPOSE. A preferential loss of ERG b-wave was detected in a substrain of C57BL/10 mice. Electroretinographic and histologic techniques were used to investigate this hitherto unknown retinopathy.

METHODS. ERGs were obtained from normal and affected C57BL/10. C57BL/6 mice served also as controls. a-Wave and c-wave analyses were performed. Microscopic investigations were done at two different ages.

RESULTS. In the scotopic ERG, a severe reduction of the b-wave amplitude could be observed, whereas the a-wave was only moderately attenuated ("negative ERG"). With age, the a-wave amplitude further decreased, but the rate of reduction was comparable to normals. Oscillatory potentials were severely altered, and the photopic ERG was absent. The ERG c-wave was comparable to normal. ERG a-wave analysis also revealed a reduced maximum amplitude, but no significant difference of receptor sensitivity. Light microscopy revealed a thinning of all retinal layers but mostly of the outer plexiform layer. The number of photoreceptor nuclei was reduced by one third. Electron microscopy revealed a profound loss of ribbon-shaped synapses between rod and rod-bipolar cells and severely abnormal ribbons in the case of cones.

CONCLUSIONS. The so-called negative ERG was associated with alterations in the synaptic layer between rods and rod bipolars. The absent cone ERG may be due to the altered cone-on-bipolar synapses. The overall thinning of the retina as well as the moderately reduced scotopic a-wave amplitude remain unexplained.




This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
J. Yang, B. Pawlyk, X.-H. Wen, M. Adamian, M. Soloviev, N. Michaud, Y. Zhao, M. A. Sandberg, C. L. Makino, and T. Li
Mpp4 is required for proper localization of plasma membrane calcium ATPases and maintenance of calcium homeostasis at the rod photoreceptor synaptic terminals
Hum. Mol. Genet., May 1, 2007; 16(9): 1017 - 1029.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. A. Wycisk, B. Budde, S. Feil, S. Skosyrski, F. Buzzi, J. Neidhardt, E. Glaus, P. Nurnberg, K. Ruether, and W. Berger
Structural and Functional Abnormalities of Retinal Ribbon Synapses due to Cacna2d4 Mutation.
Invest. Ophthalmol. Vis. Sci., August 1, 2006; 47(8): 3523 - 3530.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. L. Ball, P. A. Powers, H.-S. Shin, C. W. Morgans, N. S. Peachey, and R. G. Gregg
Role of the {beta}2 Subunit of Voltage-Dependent Calcium Channels in the Retinal Outer Plexiform Layer
Invest. Ophthalmol. Vis. Sci., May 1, 2002; 43(5): 1595 - 1603.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the Association for Research in Vision and Ophthalmology