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From the Academic Department of Ophthalmology, Royal Eye Hospital, Manchester, United Kingdom.
PURPOSE. To study patterns of expression of alternatively spliced tenascin-C (TN-C) variants believed to mediate cellular activities in human corneal development.
METHODS. Serial sections of preterm, neonatal, child, and adult globes with
normal anterior segments were labeled with monoclonal antibodies to
TN-C. The antibodies included BC-4 and BC-8, which recognize epitopes
in conserved domains of TN-C and can thus detect all TN-C variants, and
BC-2, 
-A2, -A3, -IIIB, TN11, and -D, which bind to epitopes
in alternatively spliced fibronectin type III repeats of TN-C. Bound
antibodies were localized and visualized using an avidin-biotin
complex–alkaline phosphatase technique.
RESULTS. BC-4 and BC-8 showed similar patterns of staining, widely observed in
preterm corneas, less so in neonatal corneas, and restricted to the
limbus in the child and adult. BC-2, -A2, -A3, -IIIB, TN11,
and -D staining was largely localized in corneal epithelium (preterm
and neonatal), limbal epithelium, mast cells, and matrix surrounding
limbal vessels (preterm, neonatal, child, and adult).
CONCLUSIONS. TN-C may play a role in corneal development and in growth and differentiation of stem cells because it is widely expressed in the preterm cornea, less so in the neonate, and is restricted to the limbus in the child and adult. The differential patterns of expression of TN-C variants in normal corneas (preterm and neonatal), and in the limbus (preterm, neonatal, child, and adult), suggest specific roles played by each variant, and cell type–specific expression of the different variants.
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