| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 From the Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, Connecticut.
PURPOSE. Microphthalmia, a bHLH-zip transcription factor associated with the onset and maintenance of pigmentation, identifies the retinal pigment epithelial (RPE) compartment during optic vesicle and optic cup development. To determine a role for microphthalmia (mi) during eye development, the effects of an mi loss of function mutation on RPE and neural retinal were investigated in the mi/mi mouse.
METHODS. A series of embryonic and postnatal mi/mi and wild-type eyes were sectioned and labeled with neural retina– and RPE cell type–specific antibodies. Photoreceptor loss was quantified by counting the number of photoreceptor nuclei spanning the outer nuclear layer throughout postnatal retinal development.
RESULTS. Early neural retinal differentiation is not affected in the mi/mi mouse. The mi/mi ventral retinal pigment epithelial layer begins to develop normally, but does not pigment or attain a differentiated cuboidal morphology. The dorsal region of mi/mi retinal pigment epithelium expands and forms an ectopic retina, which develops all major retinal cell types along a similar time course as the wild type. After birth, mi/mi photoreceptors begin to form rosettes, outer segments fail to elongate, and over an extended time period, the retina degenerates.
CONCLUSIONS. Together these results suggest that early retinal development can proceed normally in the mi/mi mutant, but later retinal histogenesis is dependent on the presence of a differentiated retinal pigment epithelium. Most importantly, loss of mi function permits a change in cell fate from RPE to retina in the dorsal eye.
This article has been cited by other articles:
|
|
 |
|
  K. Bharti, W. Liu, T. Csermely, S. Bertuzzi, and H. Arnheiter Alternative promoter use in eye development: the complex role and regulation of the transcription factor MITF Development, March 15, 2008; 135(6): 1169 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Muller, H. Rohrer, and A. Vogel-Hopker Bone morphogenetic proteins specify the retinal pigment epithelium in the chick embryo Development, October 1, 2007; 134(19): 3483 - 3493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. G. Neilson Plasticity, Nuclear Diapause, and a Requiem for the Terminal Differentiation of Epithelia J. Am. Soc. Nephrol., July 1, 2007; 18(7): 1995 - 1998. [Full Text] [PDF]
|
|
|
|
 |
|
 N. Esumi, S. Kachi, P. A. Campochiaro, and D. J. Zack VMD2 Promoter Requires Two Proximal E-box Sites for Its Activity in Vivo and Is Regulated by the MITF-TFE Family J. Biol. Chem., January 19, 2007; 282(3): 1838 - 1850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Mui, J. W. Kim, G. Lemke, and S. Bertuzzi Vax genes ventralize the embryonic eye Genes & Dev., May 15, 2005; 19(10): 1249 - 1259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Horsford, M.-T. T. Nguyen, G. C. Sellar, R. Kothary, H. Arnheiter, and R. R. McInnes Chx10 repression of Mitf is required for the maintenance of mammalian neuroretinal identity Development, January 1, 2005; 132(1): 177 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rowan, C.-M. A. Chen, T. L. Young, D. E. Fisher, and C. L. Cepko Transdifferentiation of the retina into pigmented cells in ocular retardation mice defines a new function of the homeodomain gene Chx10 Development, October 15, 2004; 131(20): 5139 - 5152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Planque, G. Raposo, L. Leconte, O. Anezo, P. Martin, and S. Saule Microphthalmia Transcription Factor Induces Both Retinal Pigmented Epithelium and Neural Crest Melanocytes from Neuroretina Cells J. Biol. Chem., October 1, 2004; 279(40): 41911 - 41917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Baumer, T. Marquardt, A. Stoykova, D. Spieler, D. Treichel, R. Ashery-Padan, and P. Gruss Retinal pigmented epithelium determination requires the redundant activities of Pax2 and Pax6 Development, July 1, 2003; 130(13): 2903 - 2915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Martinez-Morales, V. Dolez, I. Rodrigo, R. Zaccarini, L. Leconte, P. Bovolenta, and S. Saule OTX2 Activates the Molecular Network Underlying Retina Pigment Epithelium Differentiation J. Biol. Chem., June 6, 2003; 278(24): 21721 - 21731. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-Y. Wu, M. Li, D. R. Hinton, L. Guo, S. Jiang, J. T. Wang, A. Zeng, J. B. Xie, M. Snead, C. Shuler, et al. Microphthalmia Resulting from Msx2-Induced Apoptosis in the Optic Vesicle Invest. Ophthalmol. Vis. Sci., June 1, 2003; 44(6): 2404 - 2412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhao, F.-C. Hung, J. S. Colvin, A. White, W. Dai, F. J. Lovicu, D. M. Ornitz, and P. A. Overbeek Patterning the optic neuroepithelium by FGF signaling and Ras activation Development, December 15, 2001; 128(24): 5051 - 5060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Fuhrmann, E. Levine, and T. Reh Extraocular mesenchyme patterns the optic vesicle during early eye development in the embryonic chick Development, January 11, 2000; 127(21): 4599 - 4609. [Abstract] [PDF]
|
|
|
|
|
|
M Nguyen and H Arnheiter Signaling and transcriptional regulation in early mammalian eye development: a link between FGF and MITF Development, January 8, 2000; 127(16): 3581 - 3591. [Abstract] [PDF]
|
|
|
|
|
|
N. Planque, L. Leconte, F. M. Coquelle, P. Martin, and S. Saule Specific Pax-6/Microphthalmia Transcription Factor Interactions Involve Their DNA-binding Domains and Inhibit Transcriptional Properties of Both Proteins J. Biol. Chem., July 27, 2001; 276(31): 29330 - 29337. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
