| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 From the Department of Ophthalmology and Visual Science, College of Medicine, The Catholic University of Korea; and the 2 Department of Biochemistry, School of Medicine, Kyungpook National University, Korea.
PURPOSE. In anterior polar cataracts and the fibrosis that can occur after cataract surgery, lens epithelial cells synthesize abundant extracellular matrix molecules and transdifferentiate into myofibroblast-like cells. Transforming growth factor (TGF)-ß has been implicated as a key player in these cataractous changes. The purpose of this study was to determine whether the TGF-ß–inducible gene h3 (ßig-h3) is expressed in lens epithelial cells from patients with anterior polar cataracts and to test whether ßig-h3 is induced by TGF-ß in cultured lens epithelial cells.
METHODS. Lens epithelial cells attached to the anterior capsules of human cataractous lenses and noncataractous lenses were examined for the expression of ßig-h3 mRNA and protein using reverse transcription–polymerase chain reaction and immunohistochemical analyses. The effect of TGF-ß on ßig-h3 gene expression was also tested in human lens epithelial B-3 cells using Northern and Western blot analyses.
RESULTS. ßig-h3 mRNA was not detected in lens epithelial cells from patients with clear lenses or patients with nuclear cataracts. Significant expression of mRNA for ßig-h3 was observed in lens epithelial cells from patients with anterior polar cataracts. Immunohistochemical analysis using anti–ßig-h3 antiserum indicated that ßig-h3 protein was present within the subcapsular plaques of anterior polar cataracts. Treatment of human lens epithelial B-3 cells with TGF-ß1 led to an increase in ßig-h3 mRNA and the secretion of ßig-h3 protein into the culture medium.
CONCLUSIONS. ßig-h3 may serve as a marker for anterior polar cataracts in addition
to previously known proteins, fibronectin, type I collagen, and
-smooth muscle actin. The functions of this protein in lens
pathology need to be further investigated.
This article has been cited by other articles:
|
|
 |
|
  N. Gotoh, N. R. Perdue, H. Matsushima, E. H. Sage, Q. Yan, and J. I. Clark An In Vitro Model of Posterior Capsular Opacity: SPARC and TGF-{beta}2 Minimize Epithelial-to-Mesenchymal Transition in Lens Epithelium Invest. Ophthalmol. Vis. Sci., October 1, 2007; 48(10): 4679 - 4687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Banh, P. A. Deschamps, J. Gauldie, P. A. Overbeek, J. G. Sivak, and J. A. West-Mays Lens-Specific Expression of TGF-{beta} Induces Anterior Subcapsular Cataract Formation in the Absence of Smad3. Invest. Ophthalmol. Vis. Sci., August 1, 2006; 47(8): 3450 - 3460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Hosler, S.-T. Wang-Su, and B. J. Wagner Role of the Proteasome in TGF-{beta} Signaling in Lens Epithelial Cells Invest. Ophthalmol. Vis. Sci., May 1, 2006; 47(5): 2045 - 2052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Mankame, R Hokanson, R Fudge, R Chowdhary, and D Busbee Alteration of gene expression in human cells treated with the agricultural chemical diazinon: possible interaction in fetal development Human and Experimental Toxicology, May 1, 2006; 25(5): 225 - 233. [Abstract] [PDF]
|
|
|
|
 |
|
 N. Kaminski, J. A. Belperio, P. B. Bitterman, L. Chen, S. W. Chensue, A. M.K. Choi, S. Dacic, J. H. Dauber, R. M. du Bois, J. J. Enghild, et al. Idiopathic Pulmonary Fibrosis Am. J. Respir. Cell Mol. Biol., September 1, 2003; 29(3): S1 - 105. [Full Text] [PDF]
|
|
|
|
|
|
S. Saika, T. Miyamoto, S.-i. Tanaka, T. Tanaka, I. Ishida, Y. Ohnishi, A. Ooshima, T. Ishiwata, G. Asano, T.-i. Chikama, et al. Response of Lens Epithelial Cells to Injury: Role of Lumican in Epithelial-Mesenchymal Transition Invest. Ophthalmol. Vis. Sci., May 1, 2003; 44(5): 2094 - 2102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X H Wan, E H Lee, H J Koh, J Song, E K Kim, C Y Kim, J B Lee, S-Y Kim, K Yao, and J H Lee Enhanced expression of transglutaminase 2 in anterior polar cataracts and its induction by TGF-{beta} in vitro Br. J. Ophthalmol., November 1, 2002; 86(11): 1293 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-E. Kim, R.-W. Park, J.-Y. Choi, Y.-C. Bae, K.-S. Kim, C.-K. Joo, and I.-S. Kim Molecular Properties of Wild-Type and Mutant {beta}IG-H3 Proteins Invest. Ophthalmol. Vis. Sci., March 1, 2002; 43(3): 656 - 661. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-E. Kim, S.-J. Kim, B.-H. Lee, R.-W. Park, K.-S. Kim, and I.-S. Kim Identification of Motifs for Cell Adhesion within the Repeated Domains of Transforming Growth Factor-beta -induced Gene, beta ig-h3 J. Biol. Chem., September 29, 2000; 275(40): 30907 - 30915. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
