IOVS Journal of Virology
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(Investigative Ophthalmology and Visual Science. 2000;41:2268-2272.)
© 2000 by The Association for Research in Vision and Ophthalmology, Inc.

Effect of Brimonidine on Rabbit Trabecular Meshwork Hyaluronidase Activity

Jorge Benozzi, Carolina O. Jaliffa, Francisco Firpo Lacoste, Diego Weinberg Llomovatte, María I. Keller Sarmiento and Ruth E. Rosenstein

From the Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental, Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Argentina.

PURPOSE. To study the presence of hyaluronidase activity in the rabbit trabecular meshwork and its regulation by brimonidine.

METHODS. A spectrophotometric assay that consists of the assessment of N-acetylhexosamine groups released from hyaluronic acid was used to examine hyaluronidase activity. Cyclic adenosine monophosphate (cAMP) levels were assessed by radioimmunoassay.

RESULTS. Hyaluronidase activity was detected in the rabbit trabecular meshwork. Its optimal activity was in the acid range of pH 3.8. Brimonidine significantly increased trabecular hyaluronidase–specific activity and decreased cAMP accumulation. Yohimbine significantly inhibited the effect of brimonidine on both hyaluronidase activity and cAMP accumulation.

CONCLUSIONS. The finding of endogenous hyaluronidase activity in rabbit trabecular meshwork supports the hypothesis that this tissue can metabolize its own glycosaminoglycan (GAG) products. The present results suggest, however, that the hypotensive effect of brimonidine could be mediated, at least in part, by its ability to increase GAG catabolism, probably through a cAMP-independent mechanism.




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J. Benozzi, L. P. Nahum, J. L. Campanelli, and R. E. Rosenstein
Effect of Hyaluronic Acid on Intraocular Pressure in Rats
Invest. Ophthalmol. Vis. Sci., July 1, 2002; 43(7): 2196 - 2200.
[Abstract] [Full Text] [PDF]


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