HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Labetoulle, M. Articles by Flamand, A. Search for Related Content PubMed PubMed Citation Articles by Labetoulle, M. Articles by Flamand, A.

Neuronal Propagation of HSV1 from the Oral Mucosa to the Eye

¹ From the Laboratoire de Génétique des Virus, Centre National de la Recherche Scientifique, Gif-sur Yvette, France; ² Institute of Physiology, University of Lausanne, Switzerland; and ³ Service d’Ophtalmologie, Hôpital de Bicêtre, Assistance Publique-Hôpitaux de Paris, Le Kremlin-Bicêtre, France.

PURPOSE. To identify possible neuronal pathways leading to herpetic ocular disease after primary oral infection in mice.

METHODS. The SC16 strain of herpes simplex virus (HSV)-1 (10⁶ plaque-forming units) was injected into the mucocutaneous border of the left upper lip. Animals were killed 2 to 10 days postinoculation (DPI). Spread of the virus in neural structures was studied by immunochemistry.

RESULTS. HSV1 first replicated at the site of inoculation and then at the superior cervical ganglion (at 2 DPI). The trigeminal ganglion and the facial nerve fibers were infected by 4 DPI. Infection of the ciliary body and iris occurred at 6 DPI, together with several brain stem nuclei belonging to the autonomic or sensory pathways. Between 8 and 10 DPI, the neural infection gradually cleared up, except for the ipsilateral sympathetic ganglion, and ipsilateral keratitis appeared in some animals.

CONCLUSIONS. The pattern of viral dissemination in this mouse model suggests that infection of iris and ciliary body results from transfer of virus in the superior cervical ganglion from sympathetic neurons innervating the lip to neighboring neurons innervating the anterior uvea. Later, zosteriform spread of virus from the trigeminal system may have contributed to the clinical and histologic findings.

This article has been cited by other articles:

				A. S. Bertke, A. Patel, and P. R. Krause Herpes Simplex Virus Latency-Associated Transcript Sequence Downstream of the Promoter Influences Type-Specific Reactivation and Viral Neurotropism J. Virol., June 15, 2007; 81(12): 6605 - 6613. [Abstract] [Full Text] [PDF]

				S. Maillet, T. Naas, S. Crepin, A.-M. Roque-Afonso, F. Lafay, S. Efstathiou, and M. Labetoulle Herpes Simplex Virus Type 1 Latently Infected Neurons Differentially Express Latency-Associated and ICP0 Transcripts. J. Virol., September 1, 2006; 80(18): 9310 - 9321. [Abstract] [Full Text] [PDF]

				M. Labetoulle, S. Maillet, S. Efstathiou, S. Dezelee, E. Frau, and F. Lafay HSV1 Latency Sites after Inoculation in the Lip: Assessment of their Localization and Connections to the Eye Invest. Ophthalmol. Vis. Sci., January 1, 2003; 44(1): 217 - 225. [Abstract] [Full Text] [PDF]

				G. D. Luker, J. P. Bardill, J. L. Prior, C. M. Pica, D. Piwnica-Worms, and D. A. Leib Noninvasive Bioluminescence Imaging of Herpes Simplex Virus Type 1 Infection and Therapy in Living Mice J. Virol., October 25, 2002; 76(23): 12149 - 12161. [Abstract] [Full Text] [PDF]

				S. Keijser, J. A. van Best, A. Van der Lelij, and M. J. Jager Reflex and Steady State Tears in Patients with Latent Stromal Herpetic Keratitis Invest. Ophthalmol. Vis. Sci., January 1, 2002; 43(1): 87 - 91. [Abstract] [Full Text] [PDF]

				B. C. Summers, T. P. Margolis, and D. A. Leib Herpes Simplex Virus Type 1 Corneal Infection Results in Periocular Disease by Zosteriform Spread J. Virol., June 1, 2001; 75(11): 5069 - 5075. [Abstract] [Full Text]