| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 From the William H. Havener Eye Center and 5 College of Medicine, The Ohio State University, Columbus; and 2 Departments of Pediatrics and 6 Internal Medicine, the 3 Holden Comprehensive Cancer Center, and the 4 Medical Scientist Training Program, University of Iowa, Iowa City.
PURPOSE. Somatostatin (SST) analogues have been used to treat proliferative diabetic retinopathy, pseudotumor cerebri, thyroid orbitopathy, and cystoid macular edema. There is a paucity of published data in regards to cell-specific distribution of SST receptors (SSTR) in normal human eye tissues. Gene expression for all five known SSTRs in normal human ciliary body/iris, retina, choroid, and cultured retinal pigment epithelial (RPE) cells were studied.
METHODS. mRNA was isolated from human ocular tissues (iris/ciliary body, retina, and choroid) dissected from eight pairs of normal eyes (9–62 years) and from RPE cells grown in culture. RT-PCR was done for all five SSTRs in all analyzed tissues. Immunohistochemistry for SSTR1 and SSTR2 was performed on eight pairs of normal human eyes (28–74 years) imbedded in paraffin.
RESULTS. SSTR1 to 5 genes are expressed in retina, SSTR1 and SSTR2 genes in cultured RPE cells, and SSTR1, 2, and 4 in ciliary body and choroid. SSTR1 and SSTR2 immunoreactivity (-ir) was observed on a variety of cells within all analyzed tissues including cornea, iris, trabecular meshwork, Schlemm’s canal, ciliary processes, ciliary muscle, retina, choroid, cultured RPE cells, and optic nerve.
CONCLUSIONS. SSTR genes are widely expressed in normal human eye tissues, with genes for SSTR1 and SSTR2 being the most widely expressed. Genes for all SSTRs are expressed in retina. SSTR1-ir and SSTR2-ir were observed in all analyzed ocular tissues. Detailed knowledge of SSTRs distribution and function in the human eye will result in a better understanding of their role in health and disease.
This article has been cited by other articles:
|
|
 |
|
  I. Minsel, R. Mentlein, S. Sel, Y. Diebold, L. Brauer, E. Muhlbauer, and F. P. Paulsen Somatostatin Actions via Somatostatin Receptors on the Ocular Surface Are Modulated by Inflammatory Processes Endocrinology, May 1, 2009; 150(5): 2254 - 2263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Torrisani, N. Hanoun, H. Laurell, F. Lopez, J.-J. Maoret, A. Souque, C. Susini, P. Cordelier, and L. Buscail Identification of an Upstream Promoter of the Human Somatostatin Receptor, hSSTR2, Which Is Controlled by Epigenetic Modifications Endocrinology, June 1, 2008; 149(6): 3137 - 3147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Hernandez, E. Carrasco, R. Casamitjana, R. Deulofeu, J. Garcia-Arumi, and R. Simo Somatostatin Molecular Variants in the Vitreous Fluid: A comparative study between diabetic patients with proliferative diabetic retinopathy and nondiabetic control subjects Diabetes Care, August 1, 2005; 28(8): 1941 - 1947. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Vasilaki, T. Papadaki, G. Notas, G. Kolios, N. Mastrodimou, D. Hoyer, M. Tsilimbaris, E. Kouroumalis, I. Pallikaris, and K. Thermos Effect of Somatostatin on Nitric Oxide Production in Human Retinal Pigment Epithelium Cell Cultures Invest. Ophthalmol. Vis. Sci., May 1, 2004; 45(5): 1499 - 1506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Taylor and D. G. Yee Somatostatin Is an Immunosuppressive Factor in Aqueous Humor Invest. Ophthalmol. Vis. Sci., June 1, 2003; 44(6): 2644 - 2649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ardjomand, N. Ardjomand, G. Schaffler, H. Radner, and Y. El-Shabrawi Expression of Somatostatin Receptors in Uveal Melanomas Invest. Ophthalmol. Vis. Sci., March 1, 2003; 44(3): 980 - 987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Lan, R. Kumar, N Di Girolamo, P McCluskey, and D Wakefield Expression and distribution of matrix metalloproteinases and their inhibitors in the human iris and ciliary body Br J Ophthalmol, February 1, 2003; 87(2): 208 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Simo, A. Lecube, L. Sararols, J. Garcia-Arumi, R. M. Segura, R. Casamitjana, and C. Hernandez Deficit of Somatostatin-Like Immunoreactivity in the Vitreous Fluid of Diabetic Patients: Possible role in the development of proliferative diabetic retinopathy Diabetes Care, December 1, 2002; 25(12): 2282 - 2286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Katz, D. D. Klisovic, M. S. O'Dorisio, R. Lynch, and M. Lubow Expression of Somatostatin Receptors 1 and 2 in Human Choroid Plexus and Arachnoid Granulations: Implications for Idiopathic Intracranial Hypertension Arch Ophthalmol, November 1, 2002; 120(11): 1540 - 1543. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
