IOVS European Journal of Biochemistry
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(Investigative Ophthalmology and Visual Science. 2001;42:2270-2282.)
© 2001 by The Association for Research in Vision and Ophthalmology, Inc.

Presence of Nerves and Their Receptors in Mouse and Human Conjunctival Goblet Cells

Yolanda Diebold, José D. Ríos, Robin R. Hodges, Ian Rawe and Darlene A. Dartt

From the Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts.

PURPOSE. To determine whether neural pathways for controlling goblet cell secretion are present in mouse and human conjunctiva.

METHODS. Mouse conjunctiva was homogenized and subjected to electrophoresis and Western blotting to detect PGP 9.5 (indicates nerves), muscarinic receptor subtypes (indicates parasympathetic pathway), and adrenergic receptors (indicates sympathetic pathway). Mouse eyes and human conjunctival tissue were analyzed by immunofluorescence microscopy. Antibodies to vasoactive intestinal peptide (VIP), tyrosine hydroxylase (TH), dopamine ß-hydroxylase (DBH), and muscarinic and {alpha}1- and ß-adrenergic receptor subtypes were used.

RESULTS. Western blot demonstrated PGP 9.5, M1, M2, and M3 muscarinic receptors and {alpha}1A-, ß1-, ß2-, and ß3-adrenergic receptors in mouse conjunctiva. Immunoreactivity for VIP, TH, and DBH was found adjacent to mouse and human goblet cells. M1 and M2 muscarinic receptors were identified throughout mouse conjunctiva, but M3 receptor was predominantly on goblet cells. All three muscarinic receptor subtypes were detected on goblet cells in human conjunctiva. {alpha}1A-Adrenergic receptors were found on epithelial cells and on goblet cells in mouse and human conjunctiva. ß1- and ß2-Adrenergic receptors were found on both epithelial and goblet cells in mouse conjunctiva, but not on human conjunctival cells. ß3-Adrenergic receptors were found on both epithelial and goblet cells in human conjunctiva but not on mouse conjunctival cells.

CONCLUSIONS. The following conclusions were drawn: parasympathetic nerves and M1, M2, and M3 muscarinic receptors, as well as sympathetic nerves are present on mouse and human goblet cells. The adrenergic receptors ß1 and ß2, but not {alpha}1A and ß3 are present on mouse conjunctival goblet cells, whereas {alpha}1A and ß3, but not ß1 and ß2 are present on human conjunctival goblet cells, suggesting that these nerves and receptors could activate goblet cell secretion in mouse and humans.




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