IOVS Human Reproduction
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lambert, W.
Right arrow Articles by Wordinger, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lambert, W.
Right arrow Articles by Wordinger, R. J.
(Investigative Ophthalmology and Visual Science. 2001;42:2315-2323.)
© 2001 by The Association for Research in Vision and Ophthalmology, Inc.

Neurotrophin and Neurotrophin Receptor Expression by Cells of the Human Lamina Cribrosa

Wendi Lambert1, Rajnee Agarwal1, William Howe2, Abbot F. Clark2,3 and Robert J. Wordinger1,3

3 From the North Texas Eye Research Institute and 1 Department of Pathology and Anatomy, Division of Cell Biology and Genetics, University of North Texas Health Science Center at Fort Worth; and 2 Glaucoma Research, Alcon Research, Ltd., Fort Worth, Texas.

PURPOSE. To determine whether cells and tissue from the human lamina cribrosa (LC) express neurotrophin and tyrosine kinase (trk) receptor mRNA and protein and whether these cells secrete neurotrophins.

METHODS. Synthesis of cDNA and the reverse transcription–polymerase chain reaction (RT-PCR) were conducted using total RNA obtained from well-characterized cell lines from the human LC and human optic nerve head (ONH) tissue. Immunofluorescent localization and Western blot analysis were used to evaluate neurotrophin and trk protein expression in cells and tissue from the human LC. Immunoassay systems (ELISAs) were used to detect the secretion of neurotrophins.

RESULTS. Two morphologically distinct cell types (LC cells and ONH astrocytes) were isolated and characterized from the human LC. Messenger RNA for each of the neurotrophins, three full-length trk receptors and two truncated trk receptors were detected in both cell types and in human ONH tissue. Protein for the neurotrophins and trk receptors were detected in LC cells, ONH astrocytes, and ONH tissue. Neither cell type expressed mRNA or protein for the low-affinity neurotrophin receptor p75. The secretion of neurotrophins was observed in both cell types.

CONCLUSIONS. Cells from the human LC express mRNA and protein of neurotrophins and trk receptors. In addition, cells from the LC secrete neurotrophins, which suggests that there is paracrine and/or autocrine signaling within the LC. Neurotrophin signaling within this region of the ONH may play important roles in the maintenance of the normal LC and in such diseases as glaucoma.




This article has been cited by other articles:


Home page
 J. Physiol.Home page
A. J. Weber, C. D. Harman, and S. Viswanathan
Effects of optic nerve injury, glaucoma, and neuroprotection on the survival, structure, and function of ganglion cells in the mammalian retina
J. Physiol., September 15, 2008; 586(18): 4393 - 4400.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
T. Tovar-Vidales, R. Roque, A. F. Clark, and R. J. Wordinger
Tissue Transglutaminase Expression and Activity in Normal and Glaucomatous Human Trabecular Meshwork Cells and Tissues
Invest. Ophthalmol. Vis. Sci., February 1, 2008; 49(2): 622 - 628.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. S. Zode, A. F. Clark, and R. J. Wordinger
Activation of the BMP Canonical Signaling Pathway in Human Optic Nerve Head Tissue and Isolated Optic Nerve Head Astrocytes and Lamina Cribrosa Cells
Invest. Ophthalmol. Vis. Sci., November 1, 2007; 48(11): 5058 - 5067.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
C. J. Lieven, M. J. Hoegger, C. R. Schlieve, and L. A. Levin
Retinal ganglion cell axotomy induces an increase in intracellular superoxide anion.
Invest. Ophthalmol. Vis. Sci., April 1, 2006; 47(4): 1477 - 1485.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. I. Swanson, C. R. Schlieve, C. J. Lieven, and L. A. Levin
Neuroprotective Effect of Sulfhydryl Reduction in a Rat Optic Nerve Crush Model
Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3737 - 3741.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
C. A. Taylor, M. Senchyna, J. Flanagan, E. M. Joyce, D. O. Cliche, A. N. Boone, S. Culp-Stewart, and J. E. Thompson
Role of eIF5A in TNF-{alpha}-Mediated Apoptosis of Lamina Cribrosa Cells
Invest. Ophthalmol. Vis. Sci., October 1, 2004; 45(10): 3568 - 3576.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
E. Ghinelli, J. Johansson, J. D. Rios, L.-L. Chen, D. Zoukhri, R. R. Hodges, and D. A. Dartt
Presence and Localization of Neurotrophins and Neurotrophin Receptors in Rat Lacrimal Gland
Invest. Ophthalmol. Vis. Sci., August 1, 2003; 44(8): 3352 - 3357.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
D. L. Fleenor, I.-H. Pang, and A. F. Clark
Involvement of AP-1 in Interleukin-1{alpha}-Stimulated MMP-3 Expression in Human Trabecular Meshwork Cells
Invest. Ophthalmol. Vis. Sci., August 1, 2003; 44(8): 3494 - 3501.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
G. Prasanna, R. Krishnamoorthy, A. F. Clark, R. J. Wordinger, and T. Yorio
Human Optic Nerve Head Astrocytes as a Target for Endothelin-1
Invest. Ophthalmol. Vis. Sci., August 1, 2002; 43(8): 2704 - 2713.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Association for Research in Vision and Ophthalmology