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1F Calcium Channel Subunit in the Rat Retina
From the John Curtin School of Medical Research, Australian National University, Canberra.
PURPOSE. The molecular identity of the calcium channels that mediate glutamate
release from photoreceptors is unknown. Mutations in the recently
identified, retina-specific 
1F calcium channel subunit
cause incomplete X-linked congenital stationary night blindness
(CSNB2), the phenotype of which is consistent with a defect in
neurotransmission within the retina. The purpose of this study was to
determine the cellular distribution of the 1F subunit in
the retina.
METHODS. Antibodies were raised against a unique peptide from the human
1F sequence. Rat retina sections were labeled with
affinity-purified 1F antibodies and the
immunofluorescence analyzed by confocal microscopy. The
1F staining was compared with that obtained with a
pan-1 antibody, used to reveal the distribution of known
voltage-gated calcium channels in the retina. Some sections were double
labeled for 1F and the photoreceptor synaptic ribbon
marker, bassoon.
RESULTS. Staining of retina sections with anti-1F resulted in
strong punctate labeling in the outer plexiform layer (OPL) and weak
punctate labeling in the inner plexiform layer (IPL), consistent with a
synaptic localization. Staining was also observed in the outer nuclear
layer. Within the OPL, 1F immunoreactivity was clustered
in discrete, horseshoe-shaped patches, the shape and dimensions of
which are characteristic of rod active zones. Similar structures were
labeled with the pan-1 antibody. Localization of
1F immunoreactivity to rod active zones was confirmed by
double labeling for bassoon, a component of photoreceptor synaptic
ribbons.
CONCLUSIONS. The distribution of 1F immunoreactivity in the OPL
suggests that calcium influx through 1F or
1F-like channels mediates glutamate release from rod
photoreceptors.
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