HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hara, Y. Articles by Abe, M. Search for Related Content PubMed PubMed Citation Articles by Hara, Y. Articles by Abe, M.

Immunoglobulin Heavy Chain Gene Analysis of Ocular Adnexal Extranodal Marginal Zone B-Cell Lymphoma

¹ From the Departments of Pathology and ² Ophthalmology, Fukushima Medical University School of Medicine, Fukushima-City, Japan.

PURPOSE. To clarify the cellular origin of extranodal marginal-zone B-cell lymphoma (EZML) of the mucosa-associated lymphoid tissue (MALT) type in ocular adnexa, the somatic mutation was analyzed in the immunoglobulin heavy-chain variable region (VH) gene.

METHODS. Eight cases of EZML in the orbit and four in the conjunctiva were studied. The VH genes were amplified by a seminested PCR and sequenced directly. These were compared with the closest published VH germline segments to determine the somatic mutation frequency. Intraclonal microheterogeneity, which was termed the ongoing mutation frequency in the current study, was estimated by counting the number of single nucleotide substitutions in individual clones and dividing by the total number of nucleotides analyzed. Nine cases of gastrointestinal EMZL were also examined for comparison.

RESULTS. The somatic mutation frequency varied between 2.0% and 12.7%, with a mean value of 7.9%. Ten cases with intraclonal microheterogeneity showed between one and six further substitutions. The average of ongoing mutation frequency was 0.11%, with a range of 0% to 0.25%. In the gastrointestinal EMZLs, the average of somatic mutation frequency was 8.5% (1.5%–14.2%) and of ongoing mutation frequency was 0.51% (0.25%–0.75%).

CONCLUSIONS. The average of ongoing mutation frequency in ocular adnexal EMZL was lower than that in gastrointestinal EMZL. Both ocular adnexal and gastrointestinal EMZLs are derived from postgerminal center memory B cells, but the low ongoing mutation frequencies of ocular adnexal EMZL may result from less antigen stimulation and follicular colonization in the orbit relative to gastrointestinal EMZL.

This article has been cited by other articles:

				A. J. M. Ferreri, R. Dolcetti, M.-Q. Du, C. Doglioni, A. Giordano Resti, L. S. Politi, C. De Conciliis, J. Radford, F. Bertoni, E. Zucca, et al. Ocular adnexal MALT lymphoma: an intriguing model for antigen-driven lymphomagenesis and microbial-targeted therapy Ann. Onc., May 1, 2008; 19(5): 835 - 846. [Abstract] [Full Text] [PDF]

				P. Adam, E. Haralambieva, M. Hartmann, Z. Mao, G. Ott, and A. Rosenwald Rare occurrence of IgVH gene translocations and restricted IgVH gene repertoire in ocular MALT-type lymphoma Haematologica, February 1, 2008; 93(2): 319 - 321. [Abstract] [Full Text] [PDF]

				A. J.M. Ferreri, M. Ponzoni, M. Guidoboni, C. De Conciliis, A. G. Resti, B. Mazzi, A. A. Lettini, J. Demeter, S. Dell'Oro, C. Doglioni, et al. Regression of Ocular Adnexal Lymphoma After Chlamydia Psittaci-Eradicating Antibiotic Therapy J. Clin. Oncol., August 1, 2005; 23(22): 5067 - 5073. [Abstract] [Full Text] [PDF]

				A. J. M. Ferreri, M. Guidoboni, M. Ponzoni, C. De Conciliis, S. Dell'Oro, K. Fleischhauer, L. Caggiari, A. A. Lettini, E. Dal Cin, R. Ieri, et al. Evidence for an Association Between Chlamydia psittaci and Ocular Adnexal Lymphomas J Natl Cancer Inst, April 21, 2004; 96(8): 586 - 594. [Abstract] [Full Text] [PDF]