IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alizadeh, M.
Right arrow Articles by Hjelmeland, L. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alizadeh, M.
Right arrow Articles by Hjelmeland, L. M.
(Investigative Ophthalmology and Visual Science. 2001;42:2706-2713.)
© 2001 by The Association for Research in Vision and Ophthalmology, Inc.

Downregulation of Differentiation Specific Gene Expression by Oxidative Stress in ARPE-19 Cells

Mitra Alizadeh1, Mitsumasa Wada1, Claire M. Gelfman1, James T. Handa2 and Leonard M. Hjelmeland1,2

1 From the Section of Molecular and Cellular Biology and the 2 Department of Ophthalmology, University of California, Davis.

PURPOSE. To investigate how the differentiation of ARPE-19 cells affects the relative expression of the FGFR genes in response to oxidative stress.

METHODS. After differentiation in vitro, APRE-19 cells were treated with t-butyl hydroperoxide (tBH) or hydrogen peroxide (H2O2) to induce oxidative stress. Viability and reactive oxygen intermediate (ROI) production were measured using standard assays. The mRNA expression of FGFR1, FGFR2, cellular retinaldehyde-binding protein (CRALBP), RPE65, and heme oxygenase-1 (HO-1) were measured by Northern blot analysis as a function of treatment with tBH and H2O2.

RESULTS. ARPE-19 cells were viable at all tBH concentrations tested but showed progressive loss of viability at concentrations greater than 300 µM H2O2. Differentiated ARPE-19 cells treated with tBH or H2O2 resulted in upregulation of the HO-1 and FGFR1 transcripts. The expression of RPE-differentiated specific genes, including FGFR2, CRALBP, and RPE65 mRNAs, was downregulated with tBH or H2O2 treatment.

CONCLUSIONS. Oxidative stress in differentiated ARPE-19 cells alters the expression of FGFR1, FGFR2, CRALBP, and RPE65 toward levels characteristic of the undifferentiated state. If similar changes take place in vivo, these events could alter the proliferative potential, viability, and even the function of the RPE.




This article has been cited by other articles:


Home page
 IOVSHome page
P. E. Milbury, B. Graf, J. M. Curran-Celentano, and J. B. Blumberg
Bilberry (Vaccinium myrtillus) Anthocyanins Modulate Heme Oxygenase-1 and Glutathione S-Transferase-pi Expression in ARPE-19 Cells
Invest. Ophthalmol. Vis. Sci., May 1, 2007; 48(5): 2343 - 2349.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A.-L. Glotin, A. Calipel, J.-Y. Brossas, A.-M. Faussat, J. Treton, and F. Mascarelli
Sustained versus Transient ERK1/2 Signaling Underlies the Anti- and Proapoptotic Effects of Oxidative Stress in Human RPE Cells.
Invest. Ophthalmol. Vis. Sci., October 1, 2006; 47(10): 4614 - 4623.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
R. C. Geiger, C. M. Waters, D. W. Kamp, and M. R. Glucksberg
KGF Prevents Oxygen-Mediated Damage in ARPE-19 Cells
Invest. Ophthalmol. Vis. Sci., September 1, 2005; 46(9): 3435 - 3442.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. A. Howes, Y. Liu, J. L. Dunaief, A. Milam, J. M. Frederick, A. Marks, and W. Baehr
Receptor for Advanced Glycation End Products and Age-Related Macular Degeneration
Invest. Ophthalmol. Vis. Sci., October 1, 2004; 45(10): 3713 - 3720.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
N. Strunnikova, C. Zhang, D. Teichberg, S. W. Cousins, J. Baffi, K. G. Becker, and K. G. Csaky
Survival of Retinal Pigment Epithelium after Exposure to Prolonged Oxidative Injury: A Detailed Gene Expression and Cellular Analysis
Invest. Ophthalmol. Vis. Sci., October 1, 2004; 45(10): 3767 - 3777.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. A. Deora, D. Gravotta, G. Kreitzer, J. Hu, D. Bok, and E. Rodriguez-Boulan
The Basolateral Targeting Signal of CD147 (EMMPRIN) Consists of a Single Leucine and Is Not Recognized by Retinal Pigment Epithelium
Mol. Biol. Cell, September 1, 2004; 15(9): 4148 - 4165.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. Tian, K. Ishibashi, and J. T. Handa
The expression of native and cultured RPE grown on different matrices
Physiol Genomics, April 13, 2004; 17(2): 170 - 182.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
T. A. Bailey, N. Kanuga, I. A. Romero, J. Greenwood, P. J. Luthert, and M. E. Cheetham
Oxidative Stress Affects the Junctional Integrity of Retinal Pigment Epithelial Cells
Invest. Ophthalmol. Vis. Sci., February 1, 2004; 45(2): 675 - 684.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
N. J. Philp, D. Wang, H. Yoon, and L. M. Hjelmeland
Polarized Expression of Monocarboxylate Transporters in Human Retinal Pigment Epithelium and ARPE-19 Cells
Invest. Ophthalmol. Vis. Sci., April 1, 2003; 44(4): 1716 - 1721.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Association for Research in Vision and Ophthalmology