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From 1 Unité Propre de Recherche de l’Enseignement Superieur (UPRES) EA2689, Institut National de la Santé et de la Recherche Médicale (INSERM), Institute Fédératif de Recherche (IFR) 22, Centre Hospitalier Universitaire (CHU), Lille, France; the 2 Imaging, Laser and Low Vision Rehabilitation Center, Lambersart, France; the 3 Department of Ophthalmology, Lariboisière University Hospital, Paris, France; and the 4 Department of Pathology, Centre Hospitalier Universitaire, Lille, France.
PURPOSE. To assess a biological effect induced by temperature elevation during transpupillary thermotherapy (TTT).
METHODS. Six pigmented rabbits were anesthetized, and TTT was performed on the right eye using an 810-nm diode laser installed on a slit lamp (spot size, 1.3 mm; duration, 60 seconds; power, 92–150 mW). A series of laser pulses were aimed at the posterior pole of the retina. The left eyes were used as the control. Twenty-four hours after laser irradiation, a histologic study was performed on the chorioretinal layers. Tissue samples were fixed in formalin and embedded in paraffin. A monoclonal antibody was used to detect heat shock protein (Hsp)70 immunoreactivity, followed by a biotinylated goat anti-mouse antibody, revealed by the avidin-biotin complex and the 3-amino-9-ethyl-carbazole (AEC) chromogen. Retinal structures were further identified by hematoxylin erythrosin saffron (HES) coloration.
RESULTS. The photocoagulation threshold was found to be at the 150-mW laser power. Under this threshold, Hsp70 immunostaining was the strongest at the 127-mW power, with staining of some choroidal cells, including capillary endothelial cells. No Hsp70 immunoreactivity was observed on the retina. At the 107-mW power, Hsp70 reactivity was observed only in occasional choroidal cells. At the 98-mW power, only mild, diffuse Hsp70 immunoreactivity was observed in the choroid. At the 92-mW power, as in nonirradiated eyes, no Hsp70 immunoreactivity was detected.
CONCLUSIONS. Subthreshold transpupillary 810-nm laser irradiation induces choroidal Hsp hyperexpression. This confirms that choroidal Hsp hyperexpression can be induced during TTT, as has been recently hypothesized by several investigators.
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