IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, H.
Right arrow Articles by Zarbin, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, H.
Right arrow Articles by Zarbin, M. A.
(Investigative Ophthalmology and Visual Science. 2001;42:2990-2999.)
© 2001 by The Association for Research in Vision and Ophthalmology, Inc.

Short-Term Study of Allogeneic Retinal Pigment Epithelium Transplants onto Debrided Bruch’s Membrane

Hao Wang1, Debra S. Leonard1,2, Alessandro A. Castellarin1, Itsuro Tsukahara1, Yoshihiko Ninomiya1, Fumihiko Yagi1, Noounanong Cheewatrakoolpong1,2, Ilene K. Sugino1 and Marco A. Zarbin1,2

1 From the Department of Ophthalmology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, New Jersey; and the 2 Department of Veterans Affairs, New Jersey Health Care System, East Orange, New Jersey.

PURPOSE. To investigate the survival and behavior of retinal pigment epithelium (RPE) microaggregates transplanted onto hydraulically debrided Bruch’s membrane and to compare results of using three different vehicles for cell delivery.

METHODS. RPE microaggregates obtained from male cats were transplanted onto the tapetal area of female cats after native RPE was debrided. For the control, one of three vehicles was introduced into the debridements. Each transplant or control specimen was analyzed histologically and immunohistochemically. Transplanted male RPE cells were identified by in situ labeling of the cat Y chromosome.

RESULTS. Histologically, significant numbers of condensed, darkly stained RPE nuclei were observed in all transplants compared with few TUNEL-positive RPE cells. Cellular retinaldehyde-binding protein was present up to day 7 in all RPE cells in transplants. In both transplant and control specimens, the antibody against the Ki-67 nuclear antigen labeled some RPE cells at day 3. TUNEL-positive outer nuclear layer nuclei were most frequently observed at day 1, but were much less frequent at 7 days in both transplant and control specimens.

CONCLUSIONS. Transplanted RPE appeared to retain at least some markers of differentiation up to 7 days after surgery. Some proliferation of transplanted RPE cells was also seen. Apoptotic cell death of transplanted RPE, as judged by TUNEL staining was observed rarely. RPE transplants imposed no adverse effect on the overlying retina. RPE survival appeared to be similar with each of the three vehicles for cell delivery.




This article has been cited by other articles:


Home page
 IOVSHome page
L. Cong, D. Sun, Z. Zhang, W. Jiao, L. J. Rizzolo, and S. Peng
A Novel Rabbit Model for Studying RPE Transplantation
Invest. Ophthalmol. Vis. Sci., September 1, 2008; 49(9): 4115 - 4125.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Wang, B. Lu, P. Wood, and R. D. Lund
Grafting of ARPE-19 and Schwann Cells to the Subretinal Space in RCS Rats
Invest. Ophthalmol. Vis. Sci., July 1, 2005; 46(7): 2552 - 2560.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. A. Hansen, I. K. Sugino, F. Yagi, H. Wang, I. Tsukahara, V. Gullapalli, J. Bennett, and M. A. Zarbin
Adeno-Associated Virus Encoding Green Fluorescent Protein as a Label for Retinal Pigment Epithelium
Invest. Ophthalmol. Vis. Sci., February 1, 2003; 44(2): 772 - 780.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
P. Gouras, J. Kong, and S. H. Tsang
Retinal Degeneration and RPE Transplantation in Rpe65-/- Mice
Invest. Ophthalmol. Vis. Sci., October 1, 2002; 43(10): 3307 - 3311.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Association for Research in Vision and Ophthalmology