HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kamphuis, W. Articles by Koya, E. Search for Related Content PubMed PubMed Citation Articles by Kamphuis, W. Articles by Koya, E.

Prostanoid Receptor Gene Expression Profile in Human Trabecular Meshwork: A Quantitative Real-Time PCR Approach

¹ From the Glaucoma Research Group, Netherlands Ophthalmic Research Institute (NORI)-KNAW, and the ³ Department of Molecular and Cellular Neurobiology, Research Institute of the Neurosciences Vrije Universiteit, Graduate School of the Neurosciences, Amsterdam, The Netherlands; and the ² Department of Ophthalmology, Leiden University Medical Centre, The Netherlands.

PURPOSE. To assess the expression pattern of prostanoid receptor–encoding genes in trabecular meshwork (TM) of human donor eyes.

METHODS. Disposed human donor eyes (n = 10) were obtained from the Cornea Bank, Amsterdam. The TM was dissected from the scleral tissue and homogenized in lysis buffer, and total RNA was isolated. The RNA was converted into cDNA and used as a template for noncompetitive quantitative real-time polymerase chain reaction (PCR) using green fluorescent dye to quantify the accumulation of double-stranded PCR product. Specific primers for four housekeeping genes and DP, EP₁, EP₂, EP_3, EP₄, FP, IP, and TP receptor–encoding transcripts were developed and tested for their efficiency.

RESULTS. The characterized expression profile was highly reproducible in all samples, with the EP₂ receptor–encoding transcript in the highest abundance, followed by FP, TP, IP, and EP₄ at levels that were approximately 10 to 15 times lower than that of the EP₂ subtype. DP and EP₃ were at the lowest levels, which were, on average, 45 times and 228 times lower than EP₂, respectively.

CONCLUSIONS. These data show that all prostanoid receptors are expressed at different levels in human TM tissue. Because the gene expression of the EP₂ receptor is, on average, 15 times more abundant than that of the EP₄ receptor, it may be expected that the increase in flow and cAMP levels in response to the activation of the EP receptors by application of prostaglandin E₁ (PGE₁), is primarily mediated by the EP₂ receptor. These data should be considered when designing prostanoid receptor mimetics intended to enhance the aqueous humor outflow through the TM and Schlemm’s canal.

This article has been cited by other articles:

				E. H. C. Tang and P. M. Vanhoutte Gene expression changes of prostanoid synthases in endothelial cells and prostanoid receptors in vascular smooth muscle cells caused by aging and hypertension Physiol Genomics, February 19, 2008; 32(3): 409 - 418. [Abstract] [Full Text] [PDF]

				T. Ota, M. Aihara, T. Saeki, S. Narumiya, and M. Araie The Effects of Prostaglandin Analogues on Prostanoid EP1, EP2, and EP3 Receptor-Deficient Mice. Invest. Ophthalmol. Vis. Sci., August 1, 2006; 47(8): 3395 - 3399. [Abstract] [Full Text] [PDF]

				D. Braun, R. S. Longman, and M. L. Albert A two-step induction of indoleamine 2,3 dioxygenase (IDO) activity during dendritic-cell maturation Blood, October 1, 2005; 106(7): 2375 - 2381. [Abstract] [Full Text] [PDF]