IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yokoyama, A.
Right arrow Articles by Adachi-Usami, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yokoyama, A.
Right arrow Articles by Adachi-Usami, E.
(Investigative Ophthalmology and Visual Science. 2001;42:3283-3286.)
© 2001 by The Association for Research in Vision and Ophthalmology, Inc.

Protection of Retinal Ganglion Cells from Ischemia-Reperfusion Injury by Electrically Applied Hsp27

Akiko Yokoyama1, Toshiyuki Oshitari1, Hisanari Negishi1, Mari Dezawa2, Atsushi Mizota1 and Emiko Adachi-Usami1

1 From the Department of Ophthalmology and Visual Science, Graduate School of Medicine, Chiba University, Japan; and 2 Department of Anatomy, Yokohama City University School of Medicine, Japan.

PURPOSE. To determine whether the Hsp27 protein can rescue retinal ganglion cells (RGCs) of rats from ischemia–reperfusion injury.

METHODS. Retinal ischemia was induced in rats by clamping the ophthalmic artery within the dural sheath of the optic nerve. Immediately after removing the clamp and beginning the reperfusion, Hsp27 protein solution was injected into the vitreous, and electroporation was applied. To determine whether Hsp27 entered the RGCs, anti-Hsp27 immunohistochemistry was performed. The retinal damage was evaluated by counting the number of RGCs retrogradely labeled by 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine percholorate (diI) injected into the superior colliculus, and also by comparing the ratio of TUNEL-positive to all RGCs in the RGC layer.

RESULTS. Electroporation successfully delivered Hsp27 protein into RGCs. In the Hsp27 electroinjected group, the number of RGCs 7 days after ischemia–reperfusion was significantly higher than in the control groups. The ratio of TUNEL-positive cells to all RGCs was lower in the group electroinjected with Hsp27 protein.

CONCLUSIONS. Electroporation of Hsp27 protein into RGCs increased the resistance of the RGCs to the apoptosis induced by ischemia–reperfusion injury.




This article has been cited by other articles:


Home page
 IOVSHome page
Y. Li, S. Roth, M. Laser, J.-x. Ma, and C. E. Crosson
Retinal Preconditioning and the Induction of Heat-Shock Protein 27
Invest. Ophthalmol. Vis. Sci., March 1, 2003; 44(3): 1299 - 1304.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
X. Mo, A. Yokoyama, T. Oshitari, H. Negishi, M. Dezawa, A. Mizota, and E. Adachi-Usami
Rescue of Axotomized Retinal Ganglion Cells by BDNF Gene Electroporation in Adult Rats
Invest. Ophthalmol. Vis. Sci., July 1, 2002; 43(7): 2401 - 2405.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Association for Research in Vision and Ophthalmology