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(Investigative Ophthalmology and Visual Science. 2001;42:424-428.)
© 2001 by The Association for Research in Vision and Ophthalmology, Inc.

Localization of EP1 and FP Receptors in Human Ocular Tissues by In Situ Hybridization

Partha Mukhopadhyay1,2, Lijun Bian1,2, Hulian Yin1, Parimal Bhattacherjee1 and Christopher A. Paterson1

1 From the Department of Ophthalmology and Visual Sciences, University of Louisville, Kentucky.

PURPOSE. To examine the expression and localization of EP1 and FP receptor mRNAs in normal human ocular tissues by in situ hybridization.

METHODS. Digoxigenin-labeled human EP1 and FP receptor antisense and sense riboprobes were used for in situ hybridization on paraffin sections of normal human eye tissue.

RESULTS. In situ hybridization revealed the presence of high levels of both EP1 and FP receptor mRNA transcripts in the blood vessels of iris, ciliary body, and choroid. Both the endothelial and smooth muscle cells of blood vessels demonstrated intense hybridization signals corresponding to EP1 receptor mRNA transcript. EP1 receptor hybridization signals were present in all the muscle fibers of the ciliary body. In the retina, hybridization signals for EP1 receptors were observed in photoreceptors and both nuclear layers and in ganglion cells. The hybridization signals corresponding to FP receptor transcript were similar to those of EP1 receptors in the iris tissues. In the ciliary muscle, FP receptor mRNA transcript was predominantly present in the circular muscle and in the collagenous connective tissues; no hybridization signal for this receptor was observed in the retina.

CONCLUSIONS. The wide distribution of EP1 and FP receptor mRNAs in human ocular tissues appears to be localized in the functional sites of the respective receptor agonists. Selective localization of FP receptor mRNA in the circular muscles and collagenous connective tissues of the ciliary body suggests their involvement in the increased uveoscleral outflow of aqueous humor by PGF2{alpha}.




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Copyright © 2001 by the Association for Research in Vision and Ophthalmology