IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Russell, P.
Right arrow Articles by Johnson, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Russell, P.
Right arrow Articles by Johnson, M.
(Investigative Ophthalmology and Visual Science. 2001;42:983-986.)
© 2001 by The Association for Research in Vision and Ophthalmology, Inc.

The Presence and Properties of Myocilin in the Aqueous Humor

Paul Russell1, Ernst R. Tamm2, Franz J. Grehn3, Greda Picht3 and Mark Johnson4

1 From the National Eye Institute, National Institutes of Health, Bethesda, MD; the 2 University of Erlangen-Nürnberg, Germany; the 3 University of Würzburg, Germany; and 4 Northwestern University, Evanston, Illinois.

PURPOSE. To determine whether myocilin is present in the aqueous humor (AH) and to examine certain properties of this protein.

METHODS. Human AH was obtained at the time of either glaucoma surgery or cataract extraction. Monkey AH was obtained at the time of death, and bovine aqueous was obtained from eyes delivered from an abattoir. Column chromatography was performed on aqueous samples to determine the approximate size of the myocilin present. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and western blot analysis were performed using antibody prepared against a peptide sequence in myocilin. Analysis of the bovine proteins present in AH that were retained by a microporous filter was also performed using western blot analysis.

RESULTS. By western blot analysis, myocilin was present in human, monkey, and bovine AH. The apparent molecular size of the myocilin present in the AH were greater than 250,000 Da, when quantified with a gel filtration column. Myocilin appeared to be hydrophobic and was one of the proteins that was retained on microporous filters that were obstructed by AH.

CONCLUSIONS. Myocilin is a constituent in the AH. It appears that myocilin is a hydrophobic protein that may exist in an oligomeric state or in association with other proteins. Myocilin is retained by microporous filters and may be involved in the obstruction of these filters that occurs when AH is perfused through them.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
F. Sanchez-Sanchez, F. Martinez-Redondo, J. D. Aroca-Aguilar, M. Coca-Prados, and J. Escribano
Characterization of the Intracellular Proteolytic Cleavage of Myocilin and Identification of Calpain II as a Myocilin-processing Protease
J. Biol. Chem., September 21, 2007; 282(38): 27810 - 27824.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. P. Fautsch, C. K. Bahler, A. M. Vrabel, K. G. Howell, N. Loewen, W. L. Teo, E. M. Poeschla, and D. H. Johnson
Perfusion of His-Tagged Eukaryotic Myocilin Increases Outflow Resistance in Human Anterior Segments in the Presence of Aqueous Humor
Invest. Ophthalmol. Vis. Sci., January 1, 2006; 47(1): 213 - 221.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. P. Fautsch, K. G. Howell, A. M. Vrabel, M. C. Charlesworth, D. C. Muddiman, and D. H. Johnson
Primary Trabecular Meshwork Cells Incubated in Human Aqueous Humor Differ from Cells Incubated in Serum Supplements
Invest. Ophthalmol. Vis. Sci., August 1, 2005; 46(8): 2848 - 2856.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. D. Aroca-Aguilar, F. Sanchez-Sanchez, S. Ghosh, M. Coca-Prados, and J. Escribano
Myocilin Mutations Causing Glaucoma Inhibit the Intracellular Endoproteolytic Cleavage of Myocilin between Amino Acids Arg226 and Ile227
J. Biol. Chem., June 3, 2005; 280(22): 21043 - 21051.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. Zillig, A. Wurm, F. J. Grehn, P. Russell, and E. R. Tamm
Overexpression and Properties of Wild-Type and Tyr437His Mutated Myocilin in the Eyes of Transgenic Mice
Invest. Ophthalmol. Vis. Sci., January 1, 2005; 46(1): 223 - 234.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Gobeil, M.-A. Rodrigue, S. Moisan, T. D. Nguyen, J. R. Polansky, J. Morissette, and V. Raymond
Intracellular Sequestration of Hetero-oligomers Formed by Wild-Type and Glaucoma-Causing Myocilin Mutants
Invest. Ophthalmol. Vis. Sci., October 1, 2004; 45(10): 3560 - 3567.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. Goldwich, C. R. Ethier, D. W.-H. Chan, and E. R. Tamm
Perfusion with the Olfactomedin Domain of Myocilin Does Not Affect Outflow Facility
Invest. Ophthalmol. Vis. Sci., May 1, 2003; 44(5): 1953 - 1961.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
T. Borras, T. V. Morozova, S. L. Heinsohn, R. F. Lyman, T. F. C. Mackay, and R. R. H. Anholt
Transcription Profiling in Drosophila Eyes That Overexpress the Human Glaucoma-Associated Trabecular Meshwork-Inducible Glucocorticoid Response Protein/Myocilin (TIGR/MYOC)
Genetics, February 1, 2003; 163(2): 637 - 645.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Association for Research in Vision and Ophthalmology