IOVS Clinical and Diagnostic Laboratory Immunology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Funaki, H.
Right arrow Articles by Yamamoto, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Funaki, H.
Right arrow Articles by Yamamoto, T.
(Investigative Ophthalmology and Visual Science. 2001;42:1193-1200.)
© 2001 by The Association for Research in Vision and Ophthalmology, Inc.

Expression and Localization of Angiogenic Inhibitory Factor, Chondromodulin-I, in Adult Rat Eye

Haruko Funaki1,2, Shoichi Sawaguchi3, Kiyoshi Yaoeda1,2, Yu Koyama1, Eishin Yaoita1, Shigeo Funaki2, Motohiro Shirakashi2, Yusuke Oshima4, Chisa Shukunami4, Yuji Hiraki4, Haruki Abe2 and Tadashi Yamamoto1

1 From the Department of Structural Pathology, Institute of Nephrology and the 2 Department of Ophthalmology, Faculty of Medicine, Niigata University; the 4 Department of Molecular Interaction and Tissue Engineering Institute for Frontier Medical Sciences, Kyoto University; and the 3 Department of Ophthalmology, Ryukyu University School of Medicine, Naha, Japan.

PURPOSE. To determine the role in the eye of chondromodulin (ChM)-I, which has been identified in cartilage as an angiogenic inhibitor, the expression and localization and a possible function of ChM-I were investigated.

METHODS. Expression and localization of ChM-I in rat eyes were examined by RNase protection assay and in situ hybridization and by immunostaining, using an antibody against a synthetic peptide. The effect of recombinant ChM-I on tube morphogenesis of retinal endothelial cells was examined in culture.

RESULTS. The rat ChM-I gene was determined to encode the open reading frame of 334 amino acid residues, and ChM-I mRNA was exclusively expressed in cartilage, eye, and cerebellum in rats. ChM-I mRNA expression was evident in the iris–ciliary body, retina, and scleral compartments, but not in other compartments of the eye. In situ hybridization revealed mRNA expression in the ganglion cells, inner nuclear layer cells, and pigment epithelium in the retina and in the nonpigment epithelium of the ciliary body. Immunoreactive ChM-I was present in these cells and also in the vitreous body. Western blot analysis detected an ~25-kDa band of ChM-I presumed as a secretory form in the aqueous humor and vitreous body and an ~37-kDa band as a precursor form in the retina. Recombinant human ChM-I inhibited tube morphogenesis of human retinal endothelial cells in vitro.

CONCLUSIONS. These observations indicate a potential role for ChM-I in inhibition of angiogenesis in the rat eye.




This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
D. Docheva, E. B. Hunziker, R. Fassler, and O. Brandau
Tenomodulin Is Necessary for Tenocyte Proliferation and Tendon Maturation
Mol. Cell. Biol., January 15, 2005; 25(2): 699 - 705.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Y. Oshima, C. Shukunami, J. Honda, K. Nishida, F. Tashiro, J.-i. Miyazaki, Y. Hiraki, and Y. Tano
Expression and Localization of Tenomodulin, a Transmembrane Type Chondromodulin-I-Related Angiogenesis Inhibitor, in Mouse Eyes
Invest. Ophthalmol. Vis. Sci., May 1, 2003; 44(5): 1814 - 1823.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
O. Brandau, A. Aszodi, E. B. Hunziker, P. J. Neame, D. Vestweber, and R. Fassler
Chondromodulin I Is Dispensable during Enchondral Ossification and Eye Development
Mol. Cell. Biol., September 15, 2002; 22(18): 6627 - 6635.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Association for Research in Vision and Ophthalmology