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(Investigative Ophthalmology and Visual Science. 2001;42:1600-1609.)
© 2001 by The Association for Research in Vision and Ophthalmology, Inc.

NADPH-Diaphorase Colocalization with Somatostatin Receptor Subtypes sst2A and sst2B in the Retina

Anna Vasilaki1, Robert Gardette2, Jacques Epelbaum2 and Kyriaki Thermos1

1 From the Laboratory of Pharmacology, Department of Basic Sciences, Faculty of Medicine, University of Crete, Heraclion, Greece; and 2 Institut National de la Santé et de la Recherche Médicale, U159, Paris, France.

PURPOSE. To investigate the differential localization of somatotropin release–inhibitory factor (SRIF) receptor subtypes (sst2A and sst2B) and their possible colocalization with reduced nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase in the rat and rabbit retina.

METHODS. Polyclonal antibodies raised against sst2A and sst2B receptors were applied to 10- to 14-µm cryostat sections of rat and rabbit retinas fixed in paraformaldehyde. NADPH-diaphorase reactivity was assessed histochemically. Double labeling was performed for sst2A or sst2B receptors with NADPH-diaphorase, and with markers for the cell types present in the retina (protein kinase C [PKC], tyrosine hydroxylase; [TH], calbindin, and recoverin).

RESULTS. sst2A immunoreactivity was detected in rod bipolar cells and colocalized with NADPH-diaphorase in the rabbit, but not the rat, retina. sst2B was present only in photoreceptor cells of the rat and colocalized with NADPH-diaphorase.

CONCLUSIONS. These results suggest that SRIF, acting through sst2A receptors in bipolar cells and sst2B receptors in photoreceptor cells, may affect nitric oxide function in the rabbit and rat retina.




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