HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Löfgren, S. Articles by Söderberg, P. G. Search for Related Content PubMed PubMed Citation Articles by Löfgren, S. Articles by Söderberg, P. G.

Lens Lactate Dehydrogenase Inactivation after UV-B Irradiation: An In Vivo Measure of UVR-B Penetration

From St. Erik’s Eye Hospital, Karolinska Institutet, Stockholm, Sweden.

PURPOSE. To elucidate the spatial distribution of inactivation of lactate dehydrogenase (LDH) in ultraviolet-B radiation (UVR-B)–exposed eyes. To determine the in vivo penetration depth of UVR-B in the lens.

METHODS. LDH activity in cornea and lens was investigated with an enzyme histochemical technique. Thirty rats were exposed in vivo to UVR-B of approximately 300 nm, and the eyes were enucleated and frozen at 0, 2, and 6 hours after exposure. LDH activity in frozen sections was determined quantitatively in the corneal epithelium and four different regions in the lens. UVR-B penetration depth was estimated by using a calculated Lambertian absorption coefficient.

RESULTS. The LDH activity was decreased in the cornea and the outer anterior lens cortex at all three time points. The average decrease in enzyme activity in the time range was 35% in the cornea and 20% in the outer anterior lens cortex. UVR-B inhibition of LDH was immediate and not dependent on an inflammatory reaction within the eye. Penetration depth, corresponding to 1/e² (14%) residual UVR-B intensity, was 0.45 mm.

CONCLUSIONS. UVR-B does not exhibit any significant effect on LDH activity in the major part of the lens, and this is attributed to the shallow penetration (0.45 mm) of UVR-B into the anterior parts of the lens.

This article has been cited by other articles:

				M.-B. Tessem, T. F. Bathen, S. Lofgren, O. Saether, V. Mody, L. Meyer, X. Dong, P. G. Soderberg, and A. Midelfart Biological Response in Various Compartments of the Rat Lens after In Vivo Exposure to UVR-B Analyzed by HR-MAS 1H NMR Spectroscopy Invest. Ophthalmol. Vis. Sci., December 1, 2006; 47(12): 5404 - 5411. [Abstract] [Full Text] [PDF]

				M.-B. Tessem, T. F. Bathen, J. Cejkova, and A. Midelfart Effect of UV-A and UV-B Irradiation on the Metabolic Profile of Aqueous Humor in Rabbits Analyzed by 1H NMR Spectroscopy Invest. Ophthalmol. Vis. Sci., March 1, 2005; 46(3): 776 - 781. [Abstract] [Full Text] [PDF]

				O. Risa, O. Saether, S. Lofgren, P. G. Soderberg, J. Krane, and A. Midelfart Metabolic Changes in Rat Lens after In Vivo Exposure to Ultraviolet Irradiation: Measurements by High Resolution MAS 1H NMR Spectroscopy Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1916 - 1921. [Abstract] [Full Text] [PDF]

				S. Lofgren, R. Michael, and P. G. Soderberg Impact of Age and Sex in Ultraviolet Radiation Cataract in the Rat Invest. Ophthalmol. Vis. Sci., April 1, 2003; 44(4): 1629 - 1633. [Abstract] [Full Text] [PDF]

				X. Dong, M. Ayala, S. Lofgren, and P. G. Soderberg Ultraviolet Radiation-Induced Cataract: Age and Maximum Acceptable Dose Invest. Ophthalmol. Vis. Sci., March 1, 2003; 44(3): 1150 - 1154. [Abstract] [Full Text] [PDF]

				M. Linetsky, V. G. Chemoganskiy, F. Hu, and B. J. Ortwerth Effect of UVA Light on the Activity of Several Aged Human Lens Enzymes Invest. Ophthalmol. Vis. Sci., January 1, 2003; 44(1): 264 - 274. [Abstract] [Full Text] [PDF]