IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, W.
Right arrow Articles by Kantorow, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, W.
Right arrow Articles by Kantorow, M.
(Investigative Ophthalmology and Visual Science. 2002;43:198-204.)
© 2002 by The Association for Research in Vision and Ophthalmology, Inc.

Decreased Expression of Ribosomal Proteins in Human Age-Related Cataract

WeiYan Zhang1, John Hawse1, QingLing Huang2, Nancy Sheets1, Kevin M. Miller2, Joseph Horwitz2 and Marc Kantorow1

From 1 The Department of Biology, West Virginia University, Morgantown, West Virginia; and 2 The Jules Stein Eye Institute, University of California Los Angeles Medical School, Los Angeles, California.

PURPOSE. To identify lens epithelial genes with altered expression levels in age-related human cataract.

METHODS. Epithelia from age-related cataracts and from normal lenses were microdissected and RNA was extracted. RNAs were compared for gene expression differences by RT-PCR differential display. Transcripts exhibiting altered levels of gene expression were cloned and identified by sequencing. The expression levels of identified clones were confirmed by semiquantitative RT-PCR with three separately isolated RNA preparations. Specific primers were designed and used to examine the mRNA levels of other genes important in protein synthesis.

RESULTS. Numerous transcripts exhibited altered levels of gene expression. One transcript exhibiting a decreased level of expression in cataract compared with normal lenses was identified as encoding ribosomal protein L21. Three additional ribosomal proteins, L15, L13a, and L7a, also exhibited decreased expression in cataract compared with normal human lenses. By contrast, the levels of elongation factor (EF)-1{alpha}1 and eucaryotic initiation factor (eIF)-4E remained unchanged.

CONCLUSIONS. The results provide evidence that human age-related cataract is associated with decreased expression of L21 and other ribosomal proteins. The results suggest that modulation of protein synthesis and/or other functions mediated by ribosomal proteins is associated with age-related cataract.




This article has been cited by other articles:


Home page
 IOVSHome page
R. Grewal, J. Stepczynski, R. Kelln, T. Erickson, R. Darrow, L. Barsalou, M. Patterson, D. T. Organisciak, and P. Wong
Coordinated Changes in Classes of Ribosomal Protein Gene Expression Is Associated with Light-Induced Retinal Degeneration
Invest. Ophthalmol. Vis. Sci., November 1, 2004; 45(11): 3885 - 3895.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
O. D. Slayden, K. Hettrich, R. S. Carroll, L. N. Otto, A. L. Clark, and R. M. Brenner
Estrogen Enhances Cystatin C Expression in the Macaque Vagina
J. Clin. Endocrinol. Metab., February 1, 2004; 89(2): 883 - 891.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Goswami, N. L. Sheets, J. Zavadil, B. K. Chauhan, E. P. Bottinger, V. N. Reddy, M. Kantorow, and A. Cvekl
Spectrum and Range of Oxidative Stress Responses of Human Lens Epithelial Cells to H2O2 Insult
Invest. Ophthalmol. Vis. Sci., May 1, 2003; 44(5): 2084 - 2093.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Association for Research in Vision and Ophthalmology