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(Investigative Ophthalmology and Visual Science. 2002;43:198-204.)
© 2002 by The Association for Research in Vision and Ophthalmology, Inc.

Decreased Expression of Ribosomal Proteins in Human Age-Related Cataract

WeiYan Zhang1, John Hawse1, QingLing Huang2, Nancy Sheets1, Kevin M. Miller2, Joseph Horwitz2 and Marc Kantorow1

From 1 The Department of Biology, West Virginia University, Morgantown, West Virginia; and 2 The Jules Stein Eye Institute, University of California Los Angeles Medical School, Los Angeles, California.

PURPOSE. To identify lens epithelial genes with altered expression levels in age-related human cataract.

METHODS. Epithelia from age-related cataracts and from normal lenses were microdissected and RNA was extracted. RNAs were compared for gene expression differences by RT-PCR differential display. Transcripts exhibiting altered levels of gene expression were cloned and identified by sequencing. The expression levels of identified clones were confirmed by semiquantitative RT-PCR with three separately isolated RNA preparations. Specific primers were designed and used to examine the mRNA levels of other genes important in protein synthesis.

RESULTS. Numerous transcripts exhibited altered levels of gene expression. One transcript exhibiting a decreased level of expression in cataract compared with normal lenses was identified as encoding ribosomal protein L21. Three additional ribosomal proteins, L15, L13a, and L7a, also exhibited decreased expression in cataract compared with normal human lenses. By contrast, the levels of elongation factor (EF)-1{alpha}1 and eucaryotic initiation factor (eIF)-4E remained unchanged.

CONCLUSIONS. The results provide evidence that human age-related cataract is associated with decreased expression of L21 and other ribosomal proteins. The results suggest that modulation of protein synthesis and/or other functions mediated by ribosomal proteins is associated with age-related cataract.




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Copyright © 2002 by the Association for Research in Vision and Ophthalmology