| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
and TGF-ß
1 From the Academic Unit of Ophthalmology and Orthoptics, University of Sheffield, Royal Hallamshire Hospital, the 4 Institute for Cancer Studies, University of Sheffield Medical School, and the 5 Department of Probability and Statistics, University of Sheffield, Sheffield, United Kingdom; and the 3 Division of Therapeutics, School of Medical and Surgical Sciences, University of Nottingham, Queens Medical Centre, Nottingham, United Kingdom.
PURPOSE. To investigate potential factors involved in uveal melanoma migration and invasion in vitro.
METHODS. Using a microchemotaxis chamber, the effects were studied of a range of stimulators and inhibitors on a series of 10 primary uveal melanomas and 2 uveal melanoma cell lines, by assessing invasion through an 8-µm pore membrane, precoated with an extracellular matrix solution. In addition, invasion in response to the effect of cells and conditioned media derived from the liver and other tissues was studied for one uveal melanoma culture, by using double-chambered wells, and invasion was assessed through an 8-µm pore membrane, precoated with synthetic extracellular matrix. In all instances, invading cells were counted under x400 magnification on the lower surface of the membrane. Levels of invasion were correlated with histopathologic markers of prognosis.
RESULTS. Conditioned media and cells derived from other tissues, including the liver, increased cellular invasion of the uveal melanoma cell line studied. For specific regulators, maximum stimulation of invasion was induced by hepatic growth factor (HGF), growth-related oncogene (GRO), and macrophage inflammatory protein (MIP)-1ß, whereas significant inhibition was induced by IL-1
, TGF-ß1, and TGF-ß2.
CONCLUSIONS. The primary site of metastasis in patients with uveal melanoma is the liver. For the degree of site specificity commonly seen, regulators involved in the process may be expressed at the secondary sites, promoting adhesion, migration, invasion, and proliferation of tumor cells. HGF, GRO, MIP-1ß, IL-1, TGF-ß1, and TGF-ß2 may play a significant role in regulating invasion of uveal melanoma cells.
This article has been cited by other articles:
|
|
 |
|
  H S Mudhar, E Saunders, P Rundle, I G Rennie, and K Sisley The in vivo modulatory effects of an anterior-chamber microenvironment on uveal melanoma Br J Ophthalmol, April 1, 2009; 93(4): 535 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bakalian, J.-C. Marshall, P. Logan, D. Faingold, S. Maloney, S. Di Cesare, C. Martins, B. F. Fernandes, and M. N. Burnier Jr. Molecular Pathways Mediating Liver Metastasis in Patients with Uveal Melanoma Clin. Cancer Res., February 15, 2008; 14(4): 951 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ye, D. Hu, L. Tu, X. Zhou, F. Lu, B. Wen, W. Wu, Y. Lin, Z. Zhou, and J. Qu Involvement of PI3K/Akt Signaling Pathway in Hepatocyte Growth Factor-Induced Migration of Uveal Melanoma Cells Invest. Ophthalmol. Vis. Sci., February 1, 2008; 49(2): 497 - 504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Faingold, J.-C. Marshall, E. Antecka, S. Di Cesare, A. N. Odashiro, S. Bakalian, B. F. Fernandes, and M. N. Burnier Jr. Immune Expression and Inhibition of Heat Shock Protein 90 in Uveal Melanoma Clin. Cancer Res., February 1, 2008; 14(3): 847 - 855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Torisu-Itakura, J. H. Lee, R. P. Scheri, Y. Huynh, X. Ye, R. Essner, and D. L. Morton Molecular Characterization of Inflammatory Genes in Sentinel and Nonsentinel Nodes in Melanoma Clin. Cancer Res., June 1, 2007; 13(11): 3125 - 3132. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-S. Son and K. F. Roby Interleukin-1{alpha}-Induced Chemokines in Mouse Granulosa Cells: Impact on Keratinocyte Chemoattractant Chemokine, a CXC Subfamily Mol. Endocrinol., November 1, 2006; 20(11): 2999 - 3013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. L. Woodward, I. G. Rennie, J. L. Burn, and K. Sisley A Potential Role for TGF{beta} in the Regulation of Uveal Melanoma Adhesive Interactions with the Hepatic Endothelium Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3473 - 3477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ren, B. Cao, S. Law, Y. Xie, P. Y. Lee, L. Cheung, Y. Chen, X. Huang, H. M. Chan, P. Zhao, et al. Hepatocyte Growth Factor Promotes Cancer Cell Migration and Angiogenic Factors Expression: A Prognostic Marker of Human Esophageal Squamous Cell Carcinomas Clin. Cancer Res., September 1, 2005; 11(17): 6190 - 6197. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
