IOVS Hypertension
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(Investigative Ophthalmology and Visual Science. 2002;43:3181-3189.)
© 2002 by The Association for Research in Vision and Ophthalmology, Inc.

ECM-Stimulated Signaling and Actin Reorganization in Embryonic Corneal Epithelia Are Rho Dependent

Wende R. Reenstra1, Daniel L. Orlow1 and Kathy K. H. Svoboda2,3

1 From the Department of Emergency Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts; the 2 Department of Biomedical Science, Texas A & M Health Science Center, Baylor College of Dentistry, Dallas, Texas; and the 3 Department of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas.

PURPOSE. The goal of this study was to investigate the role of the small guanosine triphosphatase (GTPase), Rho, in the corneal epithelial response to extracellular matrix (ECM) molecules. The avian corneal epithelial model was used to establish that Rho is required for actin reorganization and tyrosine phosphorylation of integrin-mediated signal pathway proteins.

METHODS. Whole embryonic corneal epithelia were isolated without the basal lamina and either transfected with Rho-specific antisense oligonucleotides or treated with Clostridium botulinum C3 exoenzyme and then stimulated with fibronectin (FN) or collagen (COL). The epithelia were evaluated for actin reorganization and protein production including Rho protein levels and tyrosine phosphorylation with Western blot analysis.

RESULTS. After an overnight transient transfection with antisense oligonucleotides, Rho protein levels were decreased more than 80%, and tyrosine phosphorylation of all integrin-mediated signal transduction proteins was decreased compared with control epithelia. Intracellular Rho distribution did not change in the presence of antisense oligonucleotides; however, the amount of immunolabeled Rho decreased. Disrupting the signaling cascade with Rho antisense also blocked FN- and COL-stimulated actin cortical mat reformation. C. botulinum C3 exoenzyme, a pharmacologic agent that specifically causes adenosine diphosphate (ADP) ribosylation and inactivation of Rho, also blocked actin reorganization and tyrosine phosphorylation. In contrast, decreasing Raf protein levels did not change FN-mediated actin reorganization or tyrosine phosphorylation.

CONCLUSIONS. Decreasing Rho protein or blocking its function inhibited ECM-stimulated actin reorganization and signal transduction, as measured by tyrosine phosphorylation.






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Copyright © 2002 by the Association for Research in Vision and Ophthalmology