| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 From the Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina.
PURPOSE. In the photic visual cycle, retinal G protein-coupled receptor (RGR) isomerizes all-trans retinal to 11-cis retinal in the retinal pigment epithelium (RPE) after illumination. It is unclear, however, how all-trans retinal, the substrate for RGR, is generated in the RPE, because no all-trans retinol dehydrogenase (atRDH) has been identified in the RPE. This study was conducted to identify the atRDH that generates all-trans retinal in the RPE.
METHODS. The full-length cDNA encoding a novel atRDH, RDH10, was cloned by PCR based on an expressed sequence tag (EST). Cellular localization was determined at the mRNA level by Northern blot analysis, RT-PCR, and in situ hybridization and at the protein level by immunohistochemistry with an antibody specific to RDH10. The activity was measured by an RDH activity assay with recombinant RDH10 expressed in COS cells.
RESULTS. The full-length RDH10 was cloned from the human, cow, and mouse. These cDNAs encode a protein of 341 amino acids and have significant sequence homology with other short-chain dehydrogenases/reductases (SDRs). The human RDH10 shares 100% and 98.6% amino acid sequence identity with the bovine and mouse proteins, respectively, suggesting a highly conserved sequence during evolution. RDH10 is predominantly expressed in the microsomal fraction of the RPE. Human RDH10 expressed in COS cells oxidized all-trans retinol to all-trans retinal. RDH10 displayed substrate specificity for all-trans retinol and preferred nicotinamide adenine dinucleotide phosphate (NADP) as the cofactor.
CONCLUSIONS. RDH10 is a novel retinol oxidase expressed in the RPE. This enzyme can generate all-trans retinal from all-trans retinol and may play an important role in the photic visual cycle.
This article has been cited by other articles:
|
|
 |
|
  I. Strate, T. H. Min, D. Iliev, and E. M. Pera Retinol dehydrogenase 10 is a feedback regulator of retinoic acid signalling during axis formation and patterning of the central nervous system Development, February 1, 2009; 136(3): 461 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. V. Belyaeva, M. P. Johnson, and N. Y. Kedishvili Kinetic Analysis of Human Enzyme RDH10 Defines the Characteristics of a Physiologically Relevant Retinol Dehydrogenase J. Biol. Chem., July 18, 2008; 283(29): 20299 - 20308. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Maeda, T. Maeda, W. Sun, H. Zhang, W. Baehr, and K. Palczewski Redundant and unique roles of retinol dehydrogenases in the mouse retina PNAS, December 4, 2007; 104(49): 19565 - 19570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zhang, P. Hu, C. R. Krois, M. A. Kane, and J. L. Napoli Altered vitamin A homeostasis and increased size and adiposity in the rdh1-null mouse FASEB J, September 1, 2007; 21(11): 2886 - 2896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. L. Sandell, B. W. Sanderson, G. Moiseyev, T. Johnson, A. Mushegian, K. Young, J.-P. Rey, J.-x. Ma, K. Staehling-Hampton, and P. A. Trainor RDH10 is essential for synthesis of embryonic retinoic acid and is required for limb, craniofacial, and organ development Genes & Dev., May 1, 2007; 21(9): 1113 - 1124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Kurth, D. A. Thompson, K. Ruther, K. L. Feathers, J. D. Chrispell, J. Schroth, C. L. McHenry, M. Schweizer, S. Skosyrski, A. Gal, et al. Targeted Disruption of the Murine Retinal Dehydrogenase Gene Rdh12 Does Not Limit Visual Cycle Function Mol. Cell. Biol., February 15, 2007; 27(4): 1370 - 1379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Szatmari, A. Pap, R. Ruhl, J.-X. Ma, P. A. Illarionov, G. S. Besra, E. Rajnavolgyi, B. Dezso, and L. Nagy PPAR{gamma} controls CD1d expression by turning on retinoic acid synthesis in developing human dendritic cells J. Exp. Med., October 2, 2006; 203(10): 2351 - 2362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Thompson, A. R. Janecke, J. Lange, K. L. Feathers, C. A. Hubner, C. L. McHenry, D. W. Stockton, G. Rammesmayer, J. R. Lupski, G. Antinolo, et al. Retinal degeneration associated with RDH12 mutations results from decreased 11-cis retinal synthesis due to disruption of the visual cycle Hum. Mol. Genet., December 15, 2005; 14(24): 3865 - 3875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Moiseyev, Y. Chen, Y. Takahashi, B. X. Wu, and J.-x. Ma RPE65 is the isomerohydrolase in the retinoid visual cycle PNAS, August 30, 2005; 102(35): 12413 - 12418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kasus-Jacobi, J. Ou, D. G. Birch, K. G. Locke, J. M. Shelton, J. A. Richardson, A. J. Murphy, D. M. Valenzuela, G. D. Yancopoulos, and A. O. Edwards Functional Characterization of Mouse RDH11 as a Retinol Dehydrogenase Involved in Dark Adaptation in Vivo J. Biol. Chem., May 27, 2005; 280(21): 20413 - 20420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. S. Kim, A. Maeda, T. Maeda, C. Heinlein, N. Kedishvili, K. Palczewski, and P. S. Nelson Delayed Dark Adaptation in 11-cis-Retinol Dehydrogenase-deficient Mice: A ROLE OF RDH11 IN VISUAL PROCESSES IN VIVO J. Biol. Chem., March 11, 2005; 280(10): 8694 - 8704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. X. Wu, G. Moiseyev, Y. Chen, B. Rohrer, R. K. Crouch, and J.-x. Ma Identification of RDH10, an All-trans Retinol Dehydrogenase, in Retinal Muller Cells Invest. Ophthalmol. Vis. Sci., November 1, 2004; 45(11): 3857 - 3862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Gao and P. Talalay Induction of phase 2 genes by sulforaphane protects retinal pigment epithelial cells against photooxidative damage PNAS, July 13, 2004; 101(28): 10446 - 10451. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
