| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 From the Prof. Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, L. V. Prasad Eye Institute, Hyderabad, India; 2 the Centre for DNA Fingerprinting and Diagnostics, Hyderabad, India; the 3 National Institute of Immunology, New Delhi, India; and the 4 Jawaharlal Nehru Centre for Advanced Science Research, Bangalore, India.
PURPOSE. To determine the possible molecular genetic defect underlying Axenfeld-Rieger anomaly (ARA) and to identify the pathogenic mutation causing this anterior segment dysgenesis in an Indian pedigree.
METHODS. The FOXC1 gene was amplified from genomic DNA of members of an ARA-affected family and control subjects using four novel sets of primers. The amplicons were directly sequenced, and the sequences were analyzed to identify the disease-causing mutation.
RESULTS. A heterozygous novel missense mutation was identified in the coding region of the FOXC1 gene in all three patients in this family. Consistent with the autosomal dominant inheritance pattern, the mutation segregated with the disease phenotype and was fully penetrant. The mutation was found in the wing region of the highly conserved forkhead domain of the FOXC1 gene and resulted in a very severe phenotype leading to blindness.
CONCLUSIONS. This is the first study to demonstrate that a mutation in the FOXC1 wing region can cause an anterior segment dysgenesis of the eye. This mutation resulted in blindness in the ARA-affected family, and the findings suggest that the FOXC1 wing region has a functional role in the normal development of the eye. Moreover, this is the first study from India to report the genetic etiology of Axenfeld-Rieger anomaly. Genotype–phenotype correlations of FOXC1 may help in establishing the disease prognosis and also in understanding the clinical and genetic heterogeneity associated with various anterior segment dysgenesis caused by this gene.
This article has been cited by other articles:
|
|
 |
|
  K. Zarbalis, J. A. Siegenthaler, Y. Choe, S. R. May, A. S. Peterson, and S. J. Pleasure Cortical dysplasia and skull defects in mice with a Foxc1 allele reveal the role of meningeal differentiation in regulating cortical development PNAS, August 28, 2007; 104(35): 14002 - 14007. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Weisschuh, P. Dressler, F. Schuettauf, C. Wolf, B. Wissinger, and E. Gramer Novel Mutations of FOXC1 and PITX2 in Patients with Axenfeld-Rieger Malformations. Invest. Ophthalmol. Vis. Sci., September 1, 2006; 47(9): 3846 - 3852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Saleem, S. Banerjee-Basu, T. C. Murphy, A. Baxevanis, and M. A. Walter Essential structural and functional determinants within the forkhead domain of FOXC1 Nucleic Acids Res., August 6, 2004; 32(14): 4182 - 4193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. C. Murphy, R. A. Saleem, T. Footz, R. Ritch, B. McGillivray, and M. A. Walter The Wing 2 Region of the FOXC1 Forkhead Domain Is Necessary for Normal DNA-Binding and Transactivation Functions Invest. Ophthalmol. Vis. Sci., August 1, 2004; 45(8): 2531 - 2538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Saleem, S. Banerjee-Basu, F. B. Berry, A. D. Baxevanis, and M. A. Walter Structural and functional analyses of disease-causing missense mutations in the forkhead domain of FOXC1 Hum. Mol. Genet., November 15, 2003; 12(22): 2993 - 3005. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
