IOVS AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mills, R. A. D.
Right arrow Articles by Williams, K. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mills, R. A. D.
Right arrow Articles by Williams, K. A.
(Investigative Ophthalmology and Visual Science. 2002;43:647-655.)
© 2002 by The Association for Research in Vision and Ophthalmology, Inc.

Effect of Immunosuppression on Outcome Measures in a Model of Rat Limbal Transplantation

Richard A. D. Mills, Douglas J. Coster and Keryn A. Williams

From the Department of Ophthalmology, Flinders University of South Australia, Adelaide, Australia.

PURPOSE. To examine the effect of immunosuppression with intramuscularly injected cyclosporine and topical corticosteroid on limbal allograft survival in a new model in the inbred rat.

METHODS. Orthotopic limbal tissue harvested from male Fischer 344 (isografts) or male Wistar-Firth donors (allografts) was sutured into superior and inferior lamellar excision sites in female recipient Fischer 344 rats. Grafts were examined clinically for at least 55 days. Superficial epithelial cells were sampled weekly, and the DNA extracted and probed for the male-specific gene Sry by polymerase chain reaction. Recipients were killed at established intervals for immunohistochemistry. Graft-recipient animals were randomly assigned to receive either intramuscular cyclosporine plus topical prednisolone phosphate or vehicle for 4 weeks from the time of transplantation.

RESULTS. Isografts survived for a median of more than 55 days. Allografts underwent clinical rejection at a median of 6 to 7 days after grafting. Acutely rejecting allografts showed a dense mononuclear infiltrate consisting of activated CD4+ and CD8+ T cells with some macrophages. Genomic Sry was usually detectable in cells sampled from the ocular surface for more than 55 days in isografts, but not beyond 7 days in allografts. Immunosuppression prolonged allograft survival significantly, as assessed clinically, but did not prolong donor cell survival on the ocular surface, as assessed by detection of genomic Sry.

CONCLUSIONS. A robust model of limbal transplantation was developed in the rat. Isografts survived for the long term, whereas allografts underwent rapid rejection. Although clinical allograft survival was prolonged to a modest extent by immunosuppression, donor cell survival on the ocular surface was not improved.




This article has been cited by other articles:


Home page
 IOVSHome page
A. R. Djalilian, S. P. Mahesh, C. A. Koch, R. B. Nussenblatt, D. Shen, Z. Zhuang, E. J. Holland, and C.-C. Chan
Survival of Donor Epithelial Cells after Limbal Stem Cell Transplantation
Invest. Ophthalmol. Vis. Sci., March 1, 2005; 46(3): 803 - 807.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. Maruyama, J. Yamada, Y. Sano, and S. Kinoshita
Th2-Biased Immune System Promotion of Allogeneic Corneal Epithelial Cell Survival after Orthotopic Limbal Transplantation
Invest. Ophthalmol. Vis. Sci., November 1, 2003; 44(11): 4736 - 4741.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Association for Research in Vision and Ophthalmology