| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 From the Research Service, Cleveland VA Medical Center, Cleveland, Ohio; 2 Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, Ohio; 3 Department of Physiology and 4 Biotechnology Center, University of Wisconsin, Madison, Wisconsin; the 5 Department of Life Science and 6 National Creative Research Initiative Center for Calcium and Learning, Pohang University of Science and Technology, Pohang, Republic of Korea; 7 Synaptic Biochemistry Group, Division of Neuroscience, The John Curtin School of Medical Research, Australian National University, Canberra, Australia; the 8 Department of Neurosciences, Case Western Reserve University, Cleveland, Ohio; and the 9 Departments of Biochemistry and Molecular Biology and 10 Ophthalmology and Visual Science, University of Louisville, Louisville, Kentucky.
PURPOSE. Mutations in the 
1F subunit of voltage-dependent calcium channels (VDCCs) have been shown to cause incomplete congenital stationary night blindness (CSNB2). The purpose of this study was to dentify which of the four ß subunits of VDCCs participates in the formation of this channel at the photoreceptor synapse and to determine how its absence affects visual processing.
METHODS. Mice without each of the four known ß subunits of VDCCs were generated by gene targeting and transgenic rescue (CNS-ß1, -ß2) or by gene targeting alone (ß3) or were obtained from a commercial provider (ß4). Retinal function and visual sensitivity were examined by electroretinography and an active avoidance behavioral test, respectively. The structure of the retina and expression of the 1F subunit were examined at the light microscopic level and by immunohistochemistry.
RESULTS. Under dark-adapted conditions, CNS-ß2–null mice had a normal ERG a-wave, but did not have a normal b-wave. In addition, these mice showed decreased sensitivity to light. Both the a- and b-waves appear normal in the CNS-ß1–, ß3-, and ß4-null mice. Histologic analyses of all four mouse lines indicated that only the CNS-ß2–null mice had altered retinal morphology. Eyes of these mice had a thinner outer plexiform layer (OPL) than eyes of control animals. In addition, the labeling pattern of the 1F subunit in the OPL was altered in CNS-ß2–null mice.
CONCLUSIONS. The normal distribution of the 1F subunit of the VDCCs in the OPL is dependent on the expression of the ß2 subunit. The expression of both of these subunits is required for normal maintenance and/or formation of the OPL and synaptic transmission.
This article has been cited by other articles:
|
|
 |
|
  A. M. Ebert, C. A. McAnelly, A. V. Handschy, R. L. Mueller, W. A. Horne, and D. M. Garrity Genomic organization, expression, and phylogenetic analysis of Ca2+ channel {beta}4 genes in 13 vertebrate species Physiol Genomics, October 8, 2008; 35(2): 133 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. McCall and R. G. Gregg Comparisons of structural and functional abnormalities in mouse b-wave mutants J. Physiol., September 15, 2008; 586(18): 4385 - 4392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Maddox, K. A. Vessey, G. L. Yarbrough, B. M. Invergo, D. R. Cantrell, S. Inayat, V. Balannik, W. L. Hicks, N. L. Hawes, S. Byers, et al. Allelic variance between GRM6 mutants, Grm6nob3 and Grm6nob4 results in differences in retinal ganglion cell visual responses J. Physiol., September 15, 2008; 586(18): 4409 - 4424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Takada, C. Vijayasarathy, Y. Zeng, S. Kjellstrom, R. A. Bush, and P. A. Sieving Synaptic Pathology in Retinoschisis Knockout (Rs1-/y) Mouse Retina and Modification by rAAV-Rs1 Gene Delivery Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3677 - 3686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Haeseleer Interaction and Colocalization of CaBP4 and Unc119 (MRG4) in Photoreceptors Invest. Ophthalmol. Vis. Sci., June 1, 2008; 49(6): 2366 - 2375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lee, A. Jimenez, G. Cui, and F. Haeseleer Phosphorylation of the Ca2+-Binding Protein CaBP4 by Protein Kinase C {zeta} in Photoreceptors J. Neurosci., November 14, 2007; 27(46): 12743 - 12754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Tuluc, G. Kern, G. J. Obermair, and B. E. Flucher Computer modeling of siRNA knockdown effects indicates an essential role of the Ca2+ channel {alpha}2{delta}-1 subunit in cardiac excitation-contraction coupling PNAS, June 26, 2007; 104(26): 11091 - 11096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yang, B. Pawlyk, X.-H. Wen, M. Adamian, M. Soloviev, N. Michaud, Y. Zhao, M. A. Sandberg, C. L. Makino, and T. Li Mpp4 is required for proper localization of plasma membrane calcium ATPases and maintenance of calcium homeostasis at the rod photoreceptor synaptic terminals Hum. Mol. Genet., May 1, 2007; 16(9): 1017 - 1029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. S. Kern, C. M. Miller, Y. Du, L. Zheng, S. Mohr, S. L. Ball, M. Kim, J. A. Jamison, and D. P. Bingaman Topical Administration of Nepafenac Inhibits Diabetes-Induced Retinal Microvascular Disease and Underlying Abnormalities of Retinal Metabolism and Physiology Diabetes, February 1, 2007; 56(2): 373 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sonkusare, M. Fraer, J. D. Marsh, and N. J. Rusch Disrupting Calcium Channel Expression To Lower Blood Pressure: New Targeting of a Well-Known Channel Mol. Interv., December 1, 2006; 6(6): 304 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Hull, K. Studholme, S. Yazulla, and H. von Gersdorff Diurnal Changes in Exocytosis and the Number of Synaptic Ribbons at Active Zones of an ON-Type Bipolar Cell Terminal J Neurophysiol, October 1, 2006; 96(4): 2025 - 2033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Weissgerber, B. Held, W. Bloch, L. Kaestner, K. R. Chien, B. K. Fleischmann, P. Lipp, V. Flockerzi, and M. Freichel Reduced Cardiac L-Type Ca2+ Current in Cav{beta}2-/- Embryos Impairs Cardiac Development and Contraction With Secondary Defects in Vascular Maturation Circ. Res., September 29, 2006; 99(7): 749 - 757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Wycisk, B. Budde, S. Feil, S. Skosyrski, F. Buzzi, J. Neidhardt, E. Glaus, P. Nurnberg, K. Ruether, and W. Berger Structural and Functional Abnormalities of Retinal Ribbon Synapses due to Cacna2d4 Mutation. Invest. Ophthalmol. Vis. Sci., August 1, 2006; 47(8): 3523 - 3530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Khosravani and G. W. Zamponi Voltage-gated calcium channels and idiopathic generalized epilepsies. Physiol Rev, July 1, 2006; 86(3): 941 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Bjartmar, A. D. Huberman, E. M. Ullian, R. C. Renteria, X. Liu, W. Xu, J. Prezioso, M. W. Susman, D. Stellwagen, C. C. Stokes, et al. Neuronal pentraxins mediate synaptic refinement in the developing visual system. J. Neurosci., June 7, 2006; 26(23): 6269 - 6281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Y. Marmorstein, J. Wu, P. McLaughlin, J. Yocom, M. O. Karl, R. Neussert, S. Wimmers, J. B. Stanton, R. G. Gregg, O. Strauss, et al. The Light Peak of the Electroretinogram Is Dependent on Voltage-gated Calcium Channels and Antagonized by Bestrophin (Best-1) J. Gen. Physiol., April 24, 2006; 127(5): 577 - 589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Maeda, J. Lem, K. Palczewski, and F. Haeseleer A Critical Role of CaBP4 in the Cone Synapse Invest. Ophthalmol. Vis. Sci., November 1, 2005; 46(11): 4320 - 4327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mansergh, N. C. Orton, J. P. Vessey, M. R. Lalonde, W. K. Stell, F. Tremblay, S. Barnes, D. E. Rancourt, and N. T. Bech-Hansen Mutation of the calcium channel gene Cacna1f disrupts calcium signaling, synaptic transmission and cellular organization in mouse retina Hum. Mol. Genet., October 15, 2005; 14(20): 3035 - 3046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hemara-Wahanui, S. Berjukow, C. I. Hope, P. K. Dearden, S.-B. Wu, J. Wilson-Wheeler, D. M. Sharp, P. Lundon-Treweek, G. M. Clover, J.-C. Hoda, et al. A CACNA1F mutation identified in an X-linked retinal disorder shifts the voltage dependence of Cav1.4 channel activation PNAS, May 24, 2005; 102(21): 7553 - 7558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Sasaki, T. Shibasaki, P. Beguin, K. Nagashima, M. Miyazaki, and S. Seino Direct Inhibition of the Interaction between {alpha}-Interaction Domain and {beta}-Interaction Domain of Voltage-dependent Ca2+ Channels by Gem J. Biol. Chem., March 11, 2005; 280(10): 9308 - 9312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Hoda, F. Zaghetto, A. Koschak, and J. Striessnig Congenital Stationary Night Blindness Type 2 Mutations S229P, G369D, L1068P, and W1440X Alter Channel Gating or Functional Expression of Cav1.4 L-type Ca2+ Channels J. Neurosci., January 5, 2005; 25(1): 252 - 259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Van Epps, M. Hayashi, L. Lucast, G. W. Stearns, J. B. Hurley, P. De Camilli, and S. E. Brockerhoff The Zebrafish nrc Mutant Reveals a Role for the Polyphosphoinositide Phosphatase Synaptojanin 1 in Cone Photoreceptor Ribbon Anchoring J. Neurosci., October 6, 2004; 24(40): 8641 - 8650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. X. Takahashi, J. Miriyala, and H. M. Colecraft Membrane-associated guanylate kinase-like properties of {beta}-subunits required for modulation of voltage-dependent Ca2+ channels PNAS, May 4, 2004; 101(18): 7193 - 7198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Batten, Y. Imanishi, T. Maeda, D. C. Tu, A. R. Moise, D. Bronson, D. Possin, R. N. Van Gelder, W. Baehr, and K. Palczewski Lecithin-retinol Acyltransferase Is Essential for Accumulation of All-trans-Retinyl Esters in the Eye and in the Liver J. Biol. Chem., March 12, 2004; 279(11): 10422 - 10432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. McRory, J. Hamid, C. J. Doering, E. Garcia, R. Parker, K. Hamming, L. Chen, M. Hildebrand, A. M. Beedle, L. Feldcamp, et al. The CACNA1F Gene Encodes an L-Type Calcium Channel with Unique Biophysical Properties and Tissue Distribution J. Neurosci., February 18, 2004; 24(7): 1707 - 1718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Baumann, A. Gerstner, X. Zong, M. Biel, and C. Wahl-Schott Functional Characterization of the L-type Ca2+ Channel Cav1.4{alpha}1 from Mouse Retina Invest. Ophthalmol. Vis. Sci., February 1, 2004; 45(2): 708 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Ball, D. Bardenstein, and K. N. Alagramam Assessment of Retinal Structure and Function in Ames Waltzer Mice Invest. Ophthalmol. Vis. Sci., September 1, 2003; 44(9): 3986 - 3992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Koschak, D. Reimer, D. Walter, J.-C. Hoda, T. Heinzle, M. Grabner, and J. Striessnig Cav1.4{alpha}1 Subunits Can Form Slowly Inactivating Dihydropyridine-Sensitive L-Type Ca2+ Channels Lacking Ca2+-Dependent Inactivation J. Neurosci., July 9, 2003; 23(14): 6041 - 6049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. X. Takahashi, S. Mittman, and H. M. Colecraft Distinctive Modulatory Effects of Five Human Auxiliary {beta}2 Subunit Splice Variants on L-Type Calcium Channel Gating Biophys. J., May 1, 2003; 84(5): 3007 - 3021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Gregg, S. Mukhopadhyay, S. I. Candille, S. L. Ball, M. T. Pardue, M. A. McCall, and N. S. Peachey Identification of the Gene and the Mutation Responsible for the Mouse nob Phenotype Invest. Ophthalmol. Vis. Sci., January 1, 2003; 44(1): 378 - 384. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
